Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection

Darshana Dadhania, Thangamani Muthukumar, Ruchuang Ding, Baogui Li, Choli Hartono, David Serur, Surya V. Seshan, Vijay K. Sharma, Sandip Kapur, Manikkam Suthanthiran

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Acute rejection (AR) and urinary tract infection (UTI) continue to plague renal transplantation. We tested the hypotheses that UTI does not increase granzyme B mRNA levels in urinary cells, and that the levels distinguish AR from UTI. We measured the levels of granzyme B mRNA in 15 urine specimens from renal allograft recipients with UTI, 29 specimens from patients with AR but without UTI, and 14 specimens from patients without AR and without UTI. We also measured transcript levels in urine specimens from 41 nontransplant individuals, 11 with UTI and 30 without UTI. UTI did not increase granzyme B mRNA levels. Granzyme B mRNA levels were lower in renal allograft recipients with UTI compared with those with AR (P<0.0001). We conclude that bacterial UTI is unlikely to confound AR diagnosis made by measurement of granzyme B mRNA levels in urinary cells.

Original languageEnglish
Pages (from-to)1752-1754
Number of pages3
JournalTransplantation
Volume75
Issue number10
DOIs
Publication statusPublished - 27 May 2003
Externally publishedYes

Fingerprint

Urinary Tract Infections
Allografts
Kidney
Granzymes
Messenger RNA
Urine
Plague
Kidney Transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection. / Dadhania, Darshana; Muthukumar, Thangamani; Ding, Ruchuang; Li, Baogui; Hartono, Choli; Serur, David; Seshan, Surya V.; Sharma, Vijay K.; Kapur, Sandip; Suthanthiran, Manikkam.

In: Transplantation, Vol. 75, No. 10, 27.05.2003, p. 1752-1754.

Research output: Contribution to journalArticle

Dadhania, D, Muthukumar, T, Ding, R, Li, B, Hartono, C, Serur, D, Seshan, SV, Sharma, VK, Kapur, S & Suthanthiran, M 2003, 'Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection', Transplantation, vol. 75, no. 10, pp. 1752-1754. https://doi.org/10.1097/01.TP.0000063931.08861.56
Dadhania, Darshana ; Muthukumar, Thangamani ; Ding, Ruchuang ; Li, Baogui ; Hartono, Choli ; Serur, David ; Seshan, Surya V. ; Sharma, Vijay K. ; Kapur, Sandip ; Suthanthiran, Manikkam. / Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection. In: Transplantation. 2003 ; Vol. 75, No. 10. pp. 1752-1754.
@article{5043c38eee3645f4b44b4d970f8181be,
title = "Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection",
abstract = "Acute rejection (AR) and urinary tract infection (UTI) continue to plague renal transplantation. We tested the hypotheses that UTI does not increase granzyme B mRNA levels in urinary cells, and that the levels distinguish AR from UTI. We measured the levels of granzyme B mRNA in 15 urine specimens from renal allograft recipients with UTI, 29 specimens from patients with AR but without UTI, and 14 specimens from patients without AR and without UTI. We also measured transcript levels in urine specimens from 41 nontransplant individuals, 11 with UTI and 30 without UTI. UTI did not increase granzyme B mRNA levels. Granzyme B mRNA levels were lower in renal allograft recipients with UTI compared with those with AR (P<0.0001). We conclude that bacterial UTI is unlikely to confound AR diagnosis made by measurement of granzyme B mRNA levels in urinary cells.",
author = "Darshana Dadhania and Thangamani Muthukumar and Ruchuang Ding and Baogui Li and Choli Hartono and David Serur and Seshan, {Surya V.} and Sharma, {Vijay K.} and Sandip Kapur and Manikkam Suthanthiran",
year = "2003",
month = "5",
day = "27",
doi = "10.1097/01.TP.0000063931.08861.56",
language = "English",
volume = "75",
pages = "1752--1754",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Molecular signatures of urinary cells distinguish acute rejection of renal allografts from urinary tract infection

AU - Dadhania, Darshana

AU - Muthukumar, Thangamani

AU - Ding, Ruchuang

AU - Li, Baogui

AU - Hartono, Choli

AU - Serur, David

AU - Seshan, Surya V.

AU - Sharma, Vijay K.

AU - Kapur, Sandip

AU - Suthanthiran, Manikkam

PY - 2003/5/27

Y1 - 2003/5/27

N2 - Acute rejection (AR) and urinary tract infection (UTI) continue to plague renal transplantation. We tested the hypotheses that UTI does not increase granzyme B mRNA levels in urinary cells, and that the levels distinguish AR from UTI. We measured the levels of granzyme B mRNA in 15 urine specimens from renal allograft recipients with UTI, 29 specimens from patients with AR but without UTI, and 14 specimens from patients without AR and without UTI. We also measured transcript levels in urine specimens from 41 nontransplant individuals, 11 with UTI and 30 without UTI. UTI did not increase granzyme B mRNA levels. Granzyme B mRNA levels were lower in renal allograft recipients with UTI compared with those with AR (P<0.0001). We conclude that bacterial UTI is unlikely to confound AR diagnosis made by measurement of granzyme B mRNA levels in urinary cells.

AB - Acute rejection (AR) and urinary tract infection (UTI) continue to plague renal transplantation. We tested the hypotheses that UTI does not increase granzyme B mRNA levels in urinary cells, and that the levels distinguish AR from UTI. We measured the levels of granzyme B mRNA in 15 urine specimens from renal allograft recipients with UTI, 29 specimens from patients with AR but without UTI, and 14 specimens from patients without AR and without UTI. We also measured transcript levels in urine specimens from 41 nontransplant individuals, 11 with UTI and 30 without UTI. UTI did not increase granzyme B mRNA levels. Granzyme B mRNA levels were lower in renal allograft recipients with UTI compared with those with AR (P<0.0001). We conclude that bacterial UTI is unlikely to confound AR diagnosis made by measurement of granzyme B mRNA levels in urinary cells.

UR - http://www.scopus.com/inward/record.url?scp=0038350649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038350649&partnerID=8YFLogxK

U2 - 10.1097/01.TP.0000063931.08861.56

DO - 10.1097/01.TP.0000063931.08861.56

M3 - Article

C2 - 12777869

AN - SCOPUS:0038350649

VL - 75

SP - 1752

EP - 1754

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 10

ER -