Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis

Valeria De Giorgi, Luigi Buonaguro, Andrea Worschech, Maria Lina Tornesello, Francesco Izzo, Francesco M. Marincola, Ena Wang, Franco M. Buonaguro

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hepatocellular carcinomas (HCCs) are a heterogeneous group of tumors that differ in risk factors and genetic alterations. In Italy, particularly Southern Italy, chronic hepatitis C virus (HCV) infection represents the main cause of HCC. Using high-density oligoarrays, we identified consistent differences in gene-expression between HCC and normal liver tissue. Expression patterns in HCC were also readily distinguishable from those associated with liver metastases. To characterize molecular events relevant to hepatocarcinogenesis and identify biomarkers for early HCC detection, gene expression profiling of 71 liver biopsies from HCV-related primary HCC and corresponding HCV-positive non-HCC hepatic tissue, as well as gastrointestinal liver metastases paired with the apparently normal peri-tumoral liver tissue, were compared to 6 liver biopsies from healthy individuals. Characteristic gene signatures were identified when normal tissue was compared with HCV-related primary HCC, corresponding HCV-positive non-HCC as well as gastrointestinal liver metastases. Pathway analysis classified the cellular and biological functions of the genes differentially expressed as related to regulation of gene expression and post-translational modification in HCV-related primary HCC; cellular Growth and Proliferation, and Cell-To-Cell Signaling and Interaction in HCV-related non HCC samples; Cellular Growth and Proliferation and Cell Cycle in metastasis. Also characteristic gene signatures were identified of HCV-HCC progression for early HCC diagnosis. Conclusions: A diagnostic molecular signature complementing conventional pathologic assessment was identified.

Original languageEnglish
Article numbere56153
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - 18 Feb 2013
Externally publishedYes

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Hepatitis C virus
hepatoma
Viruses
metastasis
Hepacivirus
Liver
Hepatocellular Carcinoma
Neoplasm Metastasis
liver
Gene expression
Tissue
Biopsy
Genes
Cell signaling
Italy
carcinoma
biopsy
Biomarkers
Cell Proliferation
chronic hepatitis C

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

De Giorgi, V., Buonaguro, L., Worschech, A., Tornesello, M. L., Izzo, F., Marincola, F. M., ... Buonaguro, F. M. (2013). Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis. PLoS One, 8(2), [e56153]. https://doi.org/10.1371/journal.pone.0056153

Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis. / De Giorgi, Valeria; Buonaguro, Luigi; Worschech, Andrea; Tornesello, Maria Lina; Izzo, Francesco; Marincola, Francesco M.; Wang, Ena; Buonaguro, Franco M.

In: PLoS One, Vol. 8, No. 2, e56153, 18.02.2013.

Research output: Contribution to journalArticle

De Giorgi, V, Buonaguro, L, Worschech, A, Tornesello, ML, Izzo, F, Marincola, FM, Wang, E & Buonaguro, FM 2013, 'Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis', PLoS One, vol. 8, no. 2, e56153. https://doi.org/10.1371/journal.pone.0056153
De Giorgi V, Buonaguro L, Worschech A, Tornesello ML, Izzo F, Marincola FM et al. Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis. PLoS One. 2013 Feb 18;8(2). e56153. https://doi.org/10.1371/journal.pone.0056153
De Giorgi, Valeria ; Buonaguro, Luigi ; Worschech, Andrea ; Tornesello, Maria Lina ; Izzo, Francesco ; Marincola, Francesco M. ; Wang, Ena ; Buonaguro, Franco M. / Molecular Signatures Associated with HCV-Induced Hepatocellular Carcinoma and Liver Metastasis. In: PLoS One. 2013 ; Vol. 8, No. 2.
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