Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis.

Hilal A. Lashuel, Joseph S. Wall

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Electron microscopy (EM) has played a central role in our current understanding of the mechanisms underlying the pathogenesis of several amyloid diseases, including Alzheimer's disease, Parkinson's disease, and prion diseases. In this chapter, we discuss the application of various EM techniques to monitor and characterize quaternary structural changes during amyloid fibril formation in vitro and the potential of extending some of these techniques to characterizing ex vivo material. In particular, we would like to bring to the attention of the reader two very powerful molecular EM techniques that remain under utilized by researchers in the amyloid community, namely scanning transmission electron microscopy and single particle molecular averaging EM. An overview of the strength and limitations of these techniques as tools for elucidating the structural basis of amyloid fibril formation will be presented.

Original languageEnglish
Pages (from-to)81-101
Number of pages21
JournalMethods in molecular biology (Clifton, N.J.)
Volume299
Publication statusPublished - 4 Aug 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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