Molecular distinctions between Aurora A and B

A single residue change transforms Aurora A into correctly localized and functional Aurora B

Fabienne Hans, Dimitrios A. Skoufias, Stefan Dimitrov, Robert L. Margolis

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Aurora A and Aurora B, paralogue mitotic kinases, share highly similar primary sequence. Both are important to mitotic progression, but their localizations and functions are distinct. We have combined shRNA suppression with overexpression of Aurora mutants to address the cause of the distinction between Aurora A and Aurora B. Aurora A residue glycine 198 (G198), mutated to asparagine to mimic the aligned asparagine 142 (N142) of Aurora B, causes Aurora A to bind the Aurora B binding partner INCENP but not the Aurora A binding partner TPX2. The mutant Aurora A rescues Aurora B mitotic function. We conclude that binding to INCENP is alone critical to the distinct function of Aurora B. Although G198 of Aurora A is required for TPX2 binding, N142G Aurora B retains INCENP binding and Aurora B function. Thus, although a single residue change transforms Aurora A, the reciprocal mutation of Aurora B does not create Aurora A function. An Aurora A-Δ120 N-terminal truncation construct reinforces Aurora A similarity to Aurora B, because it does not associate with centrosomes but instead associates with kinetochores.

Original languageEnglish
Pages (from-to)3491-3502
Number of pages12
JournalMolecular Biology of the Cell
Volume20
Issue number15
DOIs
Publication statusPublished - 1 Aug 2009
Externally publishedYes

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Asparagine
Glycine
Kinetochores
Centrosome
Small Interfering RNA
Phosphotransferases
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Molecular distinctions between Aurora A and B : A single residue change transforms Aurora A into correctly localized and functional Aurora B. / Hans, Fabienne; Skoufias, Dimitrios A.; Dimitrov, Stefan; Margolis, Robert L.

In: Molecular Biology of the Cell, Vol. 20, No. 15, 01.08.2009, p. 3491-3502.

Research output: Contribution to journalArticle

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