Molecular characterization of the Dot/Icm-translocated AnkH and AnkJ eukaryotic-like effectors of Legionella pneumophila

Fabien Habyarimana, Chris T. Price, Marina Santic, Souhaila M. Al Khodor, Yousef Abu Kwaik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although most Dot/Icm-translocated effectors of Legionella pneumophila are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of L. pneumophila. The ankH and ankJ mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the ankH or ankJ mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell.

Original languageEnglish
Pages (from-to)1123-1134
Number of pages12
JournalInfection and Immunity
Volume78
Issue number3
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

Fingerprint

Legionella pneumophila
HEK293 Cells
Ankyrins
Transport Vesicles
Golgi Apparatus
Endoplasmic Reticulum
Cytosol
Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Molecular characterization of the Dot/Icm-translocated AnkH and AnkJ eukaryotic-like effectors of Legionella pneumophila. / Habyarimana, Fabien; Price, Chris T.; Santic, Marina; Al Khodor, Souhaila M.; Kwaik, Yousef Abu.

In: Infection and Immunity, Vol. 78, No. 3, 03.2010, p. 1123-1134.

Research output: Contribution to journalArticle

Habyarimana, Fabien ; Price, Chris T. ; Santic, Marina ; Al Khodor, Souhaila M. ; Kwaik, Yousef Abu. / Molecular characterization of the Dot/Icm-translocated AnkH and AnkJ eukaryotic-like effectors of Legionella pneumophila. In: Infection and Immunity. 2010 ; Vol. 78, No. 3. pp. 1123-1134.
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