Molecular characterization of plasmodium falciparum in Arunachal Pradesh from northeast India based on merozoite surface protein 1 & glutamate-rich protein

Nilanju Pran Sarmah, Kishore Sarma, Dibya Ranjan Bhattacharyya, Ali Sultan, Devendra Bansal, Neeru Singh, Praveen K. Bharti, Hargobinder Kaur, Rakesh Sehgal, Pradyumna Kishore Mohapatra, Jagadish Mahanta

Research output: Contribution to journalArticle

Abstract

Background & objectives: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. Methods: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. Results: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63%), followed by MAD20 (29%) and K1 (8%) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. Interpretation & conclusions: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalIndian Journal of Medical Research
Volume146
Issue numberSeptember
DOIs
Publication statusPublished - 1 Sep 2017

Fingerprint

Merozoite Surface Protein 1
Plasmodium falciparum
India
Glutamic Acid
Alleles
Proteins
Malaria
Parasites
Polymerase chain reaction
Biomarkers
Polymorphism
Falciparum Malaria
Plasticity
Blood
Genes
Fever
Genotype
DNA
Morbidity
Polymerase Chain Reaction

Keywords

  • Genetic diversity
  • Glutamate-rich protein
  • Merozoite surface protein 1
  • North East India
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Molecular characterization of plasmodium falciparum in Arunachal Pradesh from northeast India based on merozoite surface protein 1 & glutamate-rich protein. / Sarmah, Nilanju Pran; Sarma, Kishore; Bhattacharyya, Dibya Ranjan; Sultan, Ali; Bansal, Devendra; Singh, Neeru; Bharti, Praveen K.; Kaur, Hargobinder; Sehgal, Rakesh; Mohapatra, Pradyumna Kishore; Mahanta, Jagadish.

In: Indian Journal of Medical Research, Vol. 146, No. September, 01.09.2017, p. 375-380.

Research output: Contribution to journalArticle

Sarmah, Nilanju Pran ; Sarma, Kishore ; Bhattacharyya, Dibya Ranjan ; Sultan, Ali ; Bansal, Devendra ; Singh, Neeru ; Bharti, Praveen K. ; Kaur, Hargobinder ; Sehgal, Rakesh ; Mohapatra, Pradyumna Kishore ; Mahanta, Jagadish. / Molecular characterization of plasmodium falciparum in Arunachal Pradesh from northeast India based on merozoite surface protein 1 & glutamate-rich protein. In: Indian Journal of Medical Research. 2017 ; Vol. 146, No. September. pp. 375-380.
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abstract = "Background & objectives: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. Methods: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. Results: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63{\%}), followed by MAD20 (29{\%}) and K1 (8{\%}) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. Interpretation & conclusions: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region.",
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AU - Sarmah, Nilanju Pran

AU - Sarma, Kishore

AU - Bhattacharyya, Dibya Ranjan

AU - Sultan, Ali

AU - Bansal, Devendra

AU - Singh, Neeru

AU - Bharti, Praveen K.

AU - Kaur, Hargobinder

AU - Sehgal, Rakesh

AU - Mohapatra, Pradyumna Kishore

AU - Mahanta, Jagadish

PY - 2017/9/1

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N2 - Background & objectives: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. Methods: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. Results: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63%), followed by MAD20 (29%) and K1 (8%) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. Interpretation & conclusions: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region.

AB - Background & objectives: Northeast (NE) India is one of the high endemic regions for malaria with a preponderance of Plasmodium falciparum, resulting in high morbidity and mortality. The P. falciparum parasite of this region showed high polymorphism in drug-resistant molecular biomarkers. However, there is a paucity of information related to merozoite surface protein 1 (msp-1) and glutamate-rich protein (glurp) which have been extensively studied in various parts of the world. The present study was, therefore, aimed at investigating the genetic diversity of P. falciparum based on msp-1 and glurp in Arunachal Pradesh, a State in NE India. Methods: Two hundred and forty nine patients with fever were screened for malaria, of whom 75 were positive for P. falciparum. Blood samples were collected from each microscopically confirmed patient. The DNA was extracted; nested polymerase chain reaction and sequencing were performed to study the genetic diversity of msp-1 (block 2) and glurp. Results: The block 2 of msp-1 gene was found to be highly polymorphic, and overall allelic distribution showed that RO33 was the dominant allele (63%), followed by MAD20 (29%) and K1 (8%) alleles. However, an extensive diversity (9 alleles and 4 genotypes) and 6-10 repeat regions exclusively of R2 type were observed in glurp. Interpretation & conclusions: The P. falciparum population of NE India was diverse which might be responsible for higher plasticity leading to the survival of the parasite and in turn to the higher endemicity of falciparum malaria of this region.

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KW - Glutamate-rich protein

KW - Merozoite surface protein 1

KW - North East India

KW - Plasmodium falciparum

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