Modulation of AT-1R/AMPK-MAPK cascade plays crucial role for the pathogenesis of diabetic cardiomyopathy in transgenic type 2 diabetic (Spontaneous Diabetic Torii) rats

Arun Lakshmanan, Meilei Harima, Vijayakumar Sukumaran, Vivian Soetikno, Rajarajan Amirthalingam Thandavarayan, Kenji Suzuki, Makoto Kodama, Masaki Nagata, Ritsuo Takagi, Kenichi Watanabe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

There are evidences that the activation of AMPK is playing pivotal role in the lipid and glucose metabolism. It has been reported that both the AMPK and angiotensin-II acts as a negative regulator for each protein. It has been well proven that the MAPK cascade could be modulated by the presence of angiotensin-II. Moreover, studies were shown that p38 MAPK stimulates glucose uptake through the AMPK activation. Therefore, we speculate and tried to demonstrate that the modulation of AT-R/MAPK pathway through AMPK might play crucial roles for the pathogenesis of diabetic cardiomyopathy, using the transgenic (Spontaneous Diabetic Torii - SDT) rats. We performed Western blot analysis for the measurement of myocardial AT-R, AMPK and MAPK cascades-related protein expressions, p67-phox and caspase-12. In addition, we employed dihydroethidium (DHE), Azan Mallory and hemotoxylin eosin (HE) staining methods to demonstrate the superoxide radical production, fibrosis and hypertrophy, respectively. The protein expressions, such as AT-1R, p-ERK1/2, p67-phox and caspase-12 were found to be significantly increased and conversely, the Ang-(1-7) mas R, Tak1, LKB1 and p-AMPKα1, p-p38 MAPK and p-JNK protein expressions were found to be considerably decreased in the SDT rats, in comparison to the normal rats. The DHE, Azan Mallory and HE stainings also revealed that the SDT rats have more superoxide radical production, fibrosis and hypertrophy, respectively than the normal rats. Taken together, it is suggested that the modulation of AT-1R/AMPK-MAPK pathway might play crucial roles for the pathogenesis of diabetic cardiomyopathy and it could become an important therapeutic target to ameliorate the diabetic cardiomyopathy.

Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalBiochemical Pharmacology
Volume83
Issue number5
DOIs
Publication statusPublished - 1 Mar 2012
Externally publishedYes

Fingerprint

Diabetic Cardiomyopathies
AMP-Activated Protein Kinases
Rats
Modulation
Caspase 12
p38 Mitogen-Activated Protein Kinases
Hematoxylin
Eosine Yellowish-(YS)
Superoxides
Angiotensin II
Hypertrophy
Proteins
Fibrosis
Chemical activation
Staining and Labeling
Glucose
Lipid Metabolism
Metabolism
Western Blotting
Lipids

Keywords

  • AMPK
  • AT-1R
  • Diabetic cardiomyopathy
  • MAPK
  • Spontaneous Diabetic Torii

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

Modulation of AT-1R/AMPK-MAPK cascade plays crucial role for the pathogenesis of diabetic cardiomyopathy in transgenic type 2 diabetic (Spontaneous Diabetic Torii) rats. / Lakshmanan, Arun; Harima, Meilei; Sukumaran, Vijayakumar; Soetikno, Vivian; Thandavarayan, Rajarajan Amirthalingam; Suzuki, Kenji; Kodama, Makoto; Nagata, Masaki; Takagi, Ritsuo; Watanabe, Kenichi.

In: Biochemical Pharmacology, Vol. 83, No. 5, 01.03.2012, p. 653-660.

Research output: Contribution to journalArticle

Lakshmanan, Arun ; Harima, Meilei ; Sukumaran, Vijayakumar ; Soetikno, Vivian ; Thandavarayan, Rajarajan Amirthalingam ; Suzuki, Kenji ; Kodama, Makoto ; Nagata, Masaki ; Takagi, Ritsuo ; Watanabe, Kenichi. / Modulation of AT-1R/AMPK-MAPK cascade plays crucial role for the pathogenesis of diabetic cardiomyopathy in transgenic type 2 diabetic (Spontaneous Diabetic Torii) rats. In: Biochemical Pharmacology. 2012 ; Vol. 83, No. 5. pp. 653-660.
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AU - Sukumaran, Vijayakumar

AU - Soetikno, Vivian

AU - Thandavarayan, Rajarajan Amirthalingam

AU - Suzuki, Kenji

AU - Kodama, Makoto

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