Modular reporter hairpin ribozymes for analyzing molecular interactions.

Hani Najafi, Michael Famulok

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Methods for the detection of biologically relevant interactions by highly precise catalytic control elements based on hairpin ribozymes, and their subsequent analysis are described. These include ribozyme design, catalytic performance in real time as a function of fluorescence signal amplification, and applications for sensing protein and nucleic acid interactions in high-throughput formats. Detailed instructions for two of our main reporter ribozyme formats that either follow repressible or inducible regulatory mechanisms are provided. We have shown that these techniques can be applied for detecting diverse target molecules including microRNAs, or protein-protein interactions. These reporter systems thus represent a general way to obtain signal-amplifying sensors for diverse applications in molecular profiling.

Original languageEnglish
Pages (from-to)237-250
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume429
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Catalytic RNA
Proteins
MicroRNAs
Nucleic Acids
Fluorescence
hairpin ribozyme

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Modular reporter hairpin ribozymes for analyzing molecular interactions. / Najafi, Hani; Famulok, Michael.

In: Methods in molecular biology (Clifton, N.J.), Vol. 429, 2008, p. 237-250.

Research output: Contribution to journalArticle

@article{3795fa221a3147dfa2529873b9dc0d09,
title = "Modular reporter hairpin ribozymes for analyzing molecular interactions.",
abstract = "Methods for the detection of biologically relevant interactions by highly precise catalytic control elements based on hairpin ribozymes, and their subsequent analysis are described. These include ribozyme design, catalytic performance in real time as a function of fluorescence signal amplification, and applications for sensing protein and nucleic acid interactions in high-throughput formats. Detailed instructions for two of our main reporter ribozyme formats that either follow repressible or inducible regulatory mechanisms are provided. We have shown that these techniques can be applied for detecting diverse target molecules including microRNAs, or protein-protein interactions. These reporter systems thus represent a general way to obtain signal-amplifying sensors for diverse applications in molecular profiling.",
author = "Hani Najafi and Michael Famulok",
year = "2008",
language = "English",
volume = "429",
pages = "237--250",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Modular reporter hairpin ribozymes for analyzing molecular interactions.

AU - Najafi, Hani

AU - Famulok, Michael

PY - 2008

Y1 - 2008

N2 - Methods for the detection of biologically relevant interactions by highly precise catalytic control elements based on hairpin ribozymes, and their subsequent analysis are described. These include ribozyme design, catalytic performance in real time as a function of fluorescence signal amplification, and applications for sensing protein and nucleic acid interactions in high-throughput formats. Detailed instructions for two of our main reporter ribozyme formats that either follow repressible or inducible regulatory mechanisms are provided. We have shown that these techniques can be applied for detecting diverse target molecules including microRNAs, or protein-protein interactions. These reporter systems thus represent a general way to obtain signal-amplifying sensors for diverse applications in molecular profiling.

AB - Methods for the detection of biologically relevant interactions by highly precise catalytic control elements based on hairpin ribozymes, and their subsequent analysis are described. These include ribozyme design, catalytic performance in real time as a function of fluorescence signal amplification, and applications for sensing protein and nucleic acid interactions in high-throughput formats. Detailed instructions for two of our main reporter ribozyme formats that either follow repressible or inducible regulatory mechanisms are provided. We have shown that these techniques can be applied for detecting diverse target molecules including microRNAs, or protein-protein interactions. These reporter systems thus represent a general way to obtain signal-amplifying sensors for diverse applications in molecular profiling.

UR - http://www.scopus.com/inward/record.url?scp=52049097365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52049097365&partnerID=8YFLogxK

M3 - Article

C2 - 18695971

AN - SCOPUS:52049097365

VL - 429

SP - 237

EP - 250

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -