Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores

Hilal A. Lashuel, Dean M. Hartley, Benjamin M. Petre, Joseph S. Wall, Martha N. Simon, Thomas Walz, Peter T. Lansbury

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

Although APP mutations associated with inherited forms of Alzheimer's disease (AD) are relatively rare, detailed studies of these mutations may prove critical for gaining important insights into the mechanism(s) and etiology of AD. Here, we present a detailed biophysical characterization of the structural properties of protofibrils formed by the Arctic variant (E22G) of amyloid-β protein (Aβ40ARC) as well as the effect of Aβ40WT on the distribution of the protofibrillar species formed by Aβ40ARC by characterizing biologically relevant mixtures of both proteins that may mimic the situation in the heterozygous patients. These studies revealed that the Arctic mutation accelerates both Aβ oligomerization and fibrillogenesis in vitro. In addition, Aβ40 ARC was observed to affect both the morphology and the size distribution of Aβ protofibrils. Electron microscopy examination of the protofibrils formed by Aβ40ARC revealed several morphologies, including: (1) relatively compact spherical particles roughly 4-5 nm in diameter; (2) annular pore-like protofibrils; (3) large spherical particles 18-25 nm in diameter; and (4) short filaments with chain-like morphology. Conversion of Aβ40ARC protofibrils to fibrils occurred more rapidly than protofibrils formed in mixed solutions of Aβ40 WT/Aβ40ARC, suggesting that co-incubation of Aβ40ARC with Aβ40WT leads to kinetic stabilization of Aβ40ARC protofibrils. An increase in the ratio of AβWT/AβMUT(Arctic), therefore, may result in the accumulation of potential neurotoxic protofibrils and acceleration of disease progression in familial Alzheimer's disease mutation carriers.

Original languageEnglish
Pages (from-to)795-808
Number of pages14
JournalJournal of Molecular Biology
Volume332
Issue number4
DOIs
Publication statusPublished - 26 Sep 2003
Externally publishedYes

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Amyloid
Alzheimer Disease
Mutation
Amyloidogenic Proteins
AIDS-Related Complex
Disease Progression
Electron Microscopy
In Vitro Techniques
Proteins

Keywords

  • Alzheimer's disease
  • Amyloid pores
  • Amyloid-β protein (Aβ)
  • Arctic mutation
  • Protofibrils

ASJC Scopus subject areas

  • Virology

Cite this

Lashuel, H. A., Hartley, D. M., Petre, B. M., Wall, J. S., Simon, M. N., Walz, T., & Lansbury, P. T. (2003). Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores. Journal of Molecular Biology, 332(4), 795-808. https://doi.org/10.1016/S0022-2836(03)00927-6

Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores. / Lashuel, Hilal A.; Hartley, Dean M.; Petre, Benjamin M.; Wall, Joseph S.; Simon, Martha N.; Walz, Thomas; Lansbury, Peter T.

In: Journal of Molecular Biology, Vol. 332, No. 4, 26.09.2003, p. 795-808.

Research output: Contribution to journalArticle

Lashuel, Hilal A. ; Hartley, Dean M. ; Petre, Benjamin M. ; Wall, Joseph S. ; Simon, Martha N. ; Walz, Thomas ; Lansbury, Peter T. / Mixtures of wild-type and a pathogenic (E22G) form of Aβ40 in vitro accumulate protofibrils, including amyloid pores. In: Journal of Molecular Biology. 2003 ; Vol. 332, No. 4. pp. 795-808.
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