MIM regulates vertebrate neural tube closure

Wei Liu, Yuko Komiya, Courtney Mezzacappa, Deepak K. Khadka, Loren Runnels, Raymond Habas

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Neural tube closure is a critical morphogenetic event that is regulated by dynamic changes in cell shape and behavior. Although previous studies have uncovered a central role for the non-canonical Wnt signaling pathway in neural tube closure, the underlying mechanism remains poorly resolved. Here, we show that the missing in metastasis (MIM; Mtss1) protein, previously identified as a Hedgehog response gene and actin and membrane remodeling protein, specifically binds to Daam1 and couples non-canonical Wnt signaling to neural tube closure. MIM binds to a conserved domain within Daam1, and this interaction is positively regulated by Wnt stimulation. Spatial expression of MIM is enriched in the anterior neural plate and neural folds, and depletion of MIM specifically inhibits anterior neural fold closure without affecting convergent extension movements or mesoderm cell fate specification. Particularly, we find that MIM is required for neural fold elevation and apical constriction along with cell polarization and elongation in both the superficial and deep layers of the anterior neural plate. The function of MIM during neural tube closure requires both its membrane-remodeling domain and its actin-binding domain. Finally, we show that the effect of MIM on neural tube closure is not due to modulation of Hedgehog signaling in the Xenopus embryo. Together, our studies define a morphogenetic pathway involving Daam1 and MIM that transduces non-canonical Wnt signaling for the cytoskeletal changes and membrane dynamics required for vertebrate neural tube closure.

Original languageEnglish
Pages (from-to)2035-2047
Number of pages13
JournalDevelopment
Volume138
Issue number10
DOIs
Publication statusPublished - May 2011
Externally publishedYes

Fingerprint

Neural Tube
Vertebrates
Neural Crest
Neural Plate
Hedgehogs
Actins
Wnt Signaling Pathway
Membranes
Cell Shape
Mesoderm
Xenopus
Constriction
Membrane Proteins
Embryonic Structures
Neoplasm Metastasis
Genes
Proteins

Keywords

  • Daam1
  • MIM
  • Mtss1
  • Neural tube closure
  • Wnt
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Liu, W., Komiya, Y., Mezzacappa, C., Khadka, D. K., Runnels, L., & Habas, R. (2011). MIM regulates vertebrate neural tube closure. Development, 138(10), 2035-2047. https://doi.org/10.1242/dev.058800

MIM regulates vertebrate neural tube closure. / Liu, Wei; Komiya, Yuko; Mezzacappa, Courtney; Khadka, Deepak K.; Runnels, Loren; Habas, Raymond.

In: Development, Vol. 138, No. 10, 05.2011, p. 2035-2047.

Research output: Contribution to journalArticle

Liu, W, Komiya, Y, Mezzacappa, C, Khadka, DK, Runnels, L & Habas, R 2011, 'MIM regulates vertebrate neural tube closure', Development, vol. 138, no. 10, pp. 2035-2047. https://doi.org/10.1242/dev.058800
Liu W, Komiya Y, Mezzacappa C, Khadka DK, Runnels L, Habas R. MIM regulates vertebrate neural tube closure. Development. 2011 May;138(10):2035-2047. https://doi.org/10.1242/dev.058800
Liu, Wei ; Komiya, Yuko ; Mezzacappa, Courtney ; Khadka, Deepak K. ; Runnels, Loren ; Habas, Raymond. / MIM regulates vertebrate neural tube closure. In: Development. 2011 ; Vol. 138, No. 10. pp. 2035-2047.
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