Microbial profile of the leachate from Mexico city's bordo poniente composting plant: An inoculum to digest organicwaste

Aixa Kari Gállego Bravo, Daniel Alejandro Salcedo Serrano, Gloria López Jiménez, Khemlal Nirmalkar, Selvasankar Murugesan, Jaime García-Mena, María Eugenia Gutiérrez Castillo, Luis Raúl Tovar Gálvez

Research output: Contribution to journalArticle

Abstract

In recent years, municipal solid waste (MSW) management has become a complex problem worldwide. Similarly, Mexico City is facing such a situation for the management and treatment of organic fraction of municipal solid waste (OFMSW). Therefore, in this work, we investigated whether leachate from the composting plant, Bordo Poniente, located in Mexico City can be used as an inoculum for the treatment of OFMSW using thermophilic anaerobic digestion (AD) with a hydraulic retention time of 30 days. We analyzed the physicochemical properties of the leachate and performed a biochemical methane potential test. Archaeal and bacterial diversity was also identified using high throughput DNA sequencing of 16S rDNA libraries. Methane yield was 0.29 m3 CH4/kg VSadded in the positive control and 0.16 m3 CH4/kg VSadded in the treatment group. The phylum, Bacteroidetes, and genus, Methanosarcina, prevailed in the leachate. However, in thermophilic conditions, the microbial communities changed, and the phylum, Firmicutes, genera, Methanoculleus, and candidate genus, vadinCA11, were dominant in the treatment group. We concluded that the leachate contains a suitable initial charge of many active bacteria and methanogenic archaea which contribute to the AD process, hence it can be used as an inoculum for the treatment of OFMSW.

Original languageEnglish
Article number2343
JournalEnergies
Volume12
Issue number12
DOIs
Publication statusPublished - 1 Jan 2019

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Keywords

  • High throughput DNA sequencing
  • Leachate
  • Methanogens
  • Microbial diversity
  • Organic fraction of municipal solid waste
  • Thermophilic anaerobic digestion

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Energy Engineering and Power Technology
  • Energy (miscellaneous)
  • Control and Optimization
  • Electrical and Electronic Engineering

Cite this

Bravo, A. K. G., Serrano, D. A. S., Jiménez, G. L., Nirmalkar, K., Murugesan, S., García-Mena, J., Castillo, M. E. G., & Gálvez, L. R. T. (2019). Microbial profile of the leachate from Mexico city's bordo poniente composting plant: An inoculum to digest organicwaste. Energies, 12(12), [2343]. https://doi.org/10.3390/en12122343