Microarrays of over 2000 hydrogels - Identification of substrates for cellular trapping and thermally triggered release

Rong Zhang, Albert Liberski, Rosario Sanchez-Martin, Mark Bradley

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.

Original languageEnglish
Pages (from-to)6193-6201
Number of pages9
JournalBiomaterials
Volume30
Issue number31
DOIs
Publication statusPublished - 1 Oct 2009
Externally publishedYes

Fingerprint

Hydrogels
Microarrays
Polymers
Cells
Hot Temperature
Substrates
Cell Line
Printing
Stem cells
Libraries
Cell Survival
Microscopes
Cell Proliferation

Keywords

  • Cellular release
  • Cellular trapping
  • Hydrogel microarrays
  • Inkjet printing
  • Stem cells
  • Thermo-responsive polymers

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Microarrays of over 2000 hydrogels - Identification of substrates for cellular trapping and thermally triggered release. / Zhang, Rong; Liberski, Albert; Sanchez-Martin, Rosario; Bradley, Mark.

In: Biomaterials, Vol. 30, No. 31, 01.10.2009, p. 6193-6201.

Research output: Contribution to journalArticle

Zhang, Rong ; Liberski, Albert ; Sanchez-Martin, Rosario ; Bradley, Mark. / Microarrays of over 2000 hydrogels - Identification of substrates for cellular trapping and thermally triggered release. In: Biomaterials. 2009 ; Vol. 30, No. 31. pp. 6193-6201.
@article{255e573a96454d3ea4ca085639c7bd03,
title = "Microarrays of over 2000 hydrogels - Identification of substrates for cellular trapping and thermally triggered release",
abstract = "In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.",
keywords = "Cellular release, Cellular trapping, Hydrogel microarrays, Inkjet printing, Stem cells, Thermo-responsive polymers",
author = "Rong Zhang and Albert Liberski and Rosario Sanchez-Martin and Mark Bradley",
year = "2009",
month = "10",
day = "1",
doi = "10.1016/j.biomaterials.2009.07.055",
language = "English",
volume = "30",
pages = "6193--6201",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "31",

}

TY - JOUR

T1 - Microarrays of over 2000 hydrogels - Identification of substrates for cellular trapping and thermally triggered release

AU - Zhang, Rong

AU - Liberski, Albert

AU - Sanchez-Martin, Rosario

AU - Bradley, Mark

PY - 2009/10/1

Y1 - 2009/10/1

N2 - In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.

AB - In this paper we describe an approach whereby over 2000 individual polymers were synthesized, in situ, on a microscope slide using inkjet printing. Subsequent biological analysis of the entire library allowed the rapid identification of specific polymers with the desired properties. Herein we demonstrate how this array of new materials could be used for the identification of polymers that allow cellular adherence, proliferation and then mild thermal release, for multiple cell lines, including mouse embryonic stem (mES) cells. The optimal, identified hydrogels were successfully scaled-up and demonstrated excellent cell viability after thermal detachment for all cell lines tested. We believe that this approach offers an avenue to the discovery of a specific thermal release polymer for every cell line.

KW - Cellular release

KW - Cellular trapping

KW - Hydrogel microarrays

KW - Inkjet printing

KW - Stem cells

KW - Thermo-responsive polymers

UR - http://www.scopus.com/inward/record.url?scp=69649103201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69649103201&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2009.07.055

DO - 10.1016/j.biomaterials.2009.07.055

M3 - Article

C2 - 19700191

AN - SCOPUS:69649103201

VL - 30

SP - 6193

EP - 6201

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 31

ER -