Metabolomics profiling of xenobiotics in elite athletes

Relevance to supplement consumption

Fatima Al-Khelaifi, Ilhame Diboun, Francesco Donati, Francesco Botrè, Mohammed Alsayrafi, Costas Georgakopoulos, Noha Yousri, Karsten Suhre, Mohamed A. Elrayess

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Supplements are widely used among elite athletes to maintain health and improve performance. Despite multiple studies investigating use of dietary supplements by athletes, a comprehensive profiling of serum supplement metabolites in elite athletes is still lacking. This study aims to analyze the presence of various xenobiotics in serum samples from elite athletes of different sports, focusing on metabolites that potentially originate from nutritional supplements. Methods: Profiling of xenobiotics in serum samples from 478 elite athletes from different sports (football, athletics, cycling, rugby, swimming, boxing and rowing) was performed using non-targeted metabolomics-based mass spectroscopy combined with ultrahigh-performance liquid chromatography. Multivariate analysis was performed using orthogonal partial least squares discriminant analysis. Differences in metabolic levels among different sport groups were identified by univariate linear models. Results: Out of the 102 detected xenobiotics, 21 were significantly different among sport groups including metabolites that potentially prolong exercise tolerance (caffeic acid), carry a nootropic effect (2-pyrrolidinone), exert a potent anti-oxidant effect (eugenol, ferulic acid 4 sulfate, thioproline, retinol), or originate from drugs for different types of injuries (ectoine, quinate). Using Gaussian graphical modelling, a metabolic network that links various sport group-associated xenobiotics was constructed to further understand their metabolic pathways. Conclusions: This pilot data provides evidence that athletes from different sports exhibit a distinct xenobiotic profile that may reflect their drug/supplement use, diet and exposure to various chemicals. Because of limitation in the study design, replication studies are warranted to confirm results in independent data sets, aiming ultimately for better assessment of dietary supplement use by athletes.

Original languageEnglish
Article number48
JournalJournal of the International Society of Sports Nutrition
Volume15
Issue number1
DOIs
Publication statusPublished - 27 Sep 2018

Fingerprint

Metabolomics
athletes
metabolomics
Xenobiotics
xenobiotics
sports
Athletes
Sports
dietary supplements
ferulic acid
Football
metabolites
Dietary Supplements
Metabolic Networks and Pathways
Serum
Quinic Acid
Boxing
Nootropic Agents
Eugenol
ultra-performance liquid chromatography

Keywords

  • Athletes
  • Metabolomics
  • Nutritional supplements
  • Sport
  • Xenobiotics

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Al-Khelaifi, F., Diboun, I., Donati, F., Botrè, F., Alsayrafi, M., Georgakopoulos, C., ... Elrayess, M. A. (2018). Metabolomics profiling of xenobiotics in elite athletes: Relevance to supplement consumption. Journal of the International Society of Sports Nutrition, 15(1), [48]. https://doi.org/10.1186/s12970-018-0254-7

Metabolomics profiling of xenobiotics in elite athletes : Relevance to supplement consumption. / Al-Khelaifi, Fatima; Diboun, Ilhame; Donati, Francesco; Botrè, Francesco; Alsayrafi, Mohammed; Georgakopoulos, Costas; Yousri, Noha; Suhre, Karsten; Elrayess, Mohamed A.

In: Journal of the International Society of Sports Nutrition, Vol. 15, No. 1, 48, 27.09.2018.

Research output: Contribution to journalArticle

Al-Khelaifi, Fatima ; Diboun, Ilhame ; Donati, Francesco ; Botrè, Francesco ; Alsayrafi, Mohammed ; Georgakopoulos, Costas ; Yousri, Noha ; Suhre, Karsten ; Elrayess, Mohamed A. / Metabolomics profiling of xenobiotics in elite athletes : Relevance to supplement consumption. In: Journal of the International Society of Sports Nutrition. 2018 ; Vol. 15, No. 1.
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