Metabolic signatures in apoptotic human cancer cell lines

Anna Halama, Gabriele Möller, Jerzy Adamski

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cancer cells have several specific metabolic features, which have been explored for targeted therapies. Agents that promote apoptosis in tumors are currently considered as a powerful tool for cancer therapeutics. The present study aimed to design a fast, reliable and robust system for metabolite measurements in cells lines to observe impact of apoptosis on the metabolome. For that purpose the NBS (newborn screen) mass spectrometry-based metabolomics assay was adapted for cell culture approach. In HEK 293 and in cancer cell lines HepG2, PC3, and MCF7 we searched for metabolic biomarkers of apoptosis differing from that of necrosis. Already nontreated cell lines revealed distinct concentrations of metabolites. Several metabolites indicative for apoptotic processes in cell culture including aspartate, glutamate, methionine, alanine, glycine, propionyl carnitine (C3-carnitine), and malonyl carnitine (C3DC-carnitine) were observed. In some cell lines metabolite changes were visible as early as 4h after apoptosis induction and preceeding the detection by caspase 3/7 assay. We demonstrated for the first time that the metabolomic signatures might be used in the tests of efficacy of agents causing apoptosis in cell culture. These signatures could be obtained in fast high-throughput screening.

Original languageEnglish
Pages (from-to)325-335
Number of pages11
JournalOMICS A Journal of Integrative Biology
Volume15
Issue number5
DOIs
Publication statusPublished - 1 May 2011
Externally publishedYes

Fingerprint

Metabolites
Cells
Carnitine
Apoptosis
Cell Line
Cell culture
Metabolomics
Cell Culture Techniques
Neoplasms
Assays
Caspase 7
Metabolome
Biomarkers
Aspartic Acid
Caspase 3
Alanine
Methionine
Mass spectrometry
Tumors
Glutamic Acid

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Molecular Medicine

Cite this

Metabolic signatures in apoptotic human cancer cell lines. / Halama, Anna; Möller, Gabriele; Adamski, Jerzy.

In: OMICS A Journal of Integrative Biology, Vol. 15, No. 5, 01.05.2011, p. 325-335.

Research output: Contribution to journalArticle

Halama, Anna ; Möller, Gabriele ; Adamski, Jerzy. / Metabolic signatures in apoptotic human cancer cell lines. In: OMICS A Journal of Integrative Biology. 2011 ; Vol. 15, No. 5. pp. 325-335.
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