Metabolic footprint of diabetes

A multiplatform metabolomics study in an epidemiological setting

Karsten Suhre, Christa Meisinger, Angela Döring, Elisabeth Altmaier, Petra Belcredi, Christian Gieger, David Chang, Michael V. Milburn, Walter E. Gall, Klaus M. Weinberger, Hans Werner Mewes, Martin Hrabé Angelis, H. Erich Wichmann, Florian Kronenberg, Jerzy Adamski, Thomas Illig

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

Background: Metabolomics is the rapidly evolving field of the comprehensive measurement of ideally all endogenous metabolites in a biological fluid. However, no single analytic technique covers the entire spectrum of the human metabolome. Here we present results from a multiplatform study, in which we investigate what kind of results can presently be obtained in the field of diabetes research when combining metabolomics data collected on a complementary set of analytical platforms in the framework of an epidemiological study. Methodology/Principal Findings: 40 individuals with self-reported diabetes and 60 controls (male, over 54 years) were randomly selected from the participants of the population-based KORA (Cooperative Health Research in the Region of Augsburg) study, representing an extensively phenotyped sample of the general German population. Concentrations of over 420 unique small molecules were determined in overnight-fasting blood using three different techniques, covering nuclear magnetic resonance and tandem mass spectrometry. Known biomarkers of diabetes could be replicated by this multiple metabolomic platform approach, including sugar metabolites (1,5-anhydroglucoitol), ketone bodies (3-hydroxybutyrate), and branched chain amino acids. In some cases, diabetes-related medication can be detected (pioglitazone, salicylic acid). Conclusions/Significance: Our study depicts the promising potential of metabolomics in diabetes research by identification of a series of known and also novel, deregulated metabolites that associate with diabetes. Key observations include perturbations of metabolic pathways linked to kidney dysfunction (3-indoxyl sulfate), lipid metabolism (glyceropho-spholipids, free fatty acids), and interaction with the gut microflora (bile acids). Our study suggests that metabolic markers hold the potential to detect diabetes-related complications already under sub-clinical conditions in the general population.

Original languageEnglish
Article numbere13953
JournalPLoS One
Volume5
Issue number11
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Metabolomics
metabolomics
Medical problems
epidemiological studies
diabetes
Epidemiologic Studies
pioglitazone
Metabolites
Indican
Research
Population
Ketone Bodies
Branched Chain Amino Acids
3-Hydroxybutyric Acid
metabolites
Salicylic Acid
Metabolome
Diabetes Complications
Tandem Mass Spectrometry
Metabolic Networks and Pathways

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Suhre, K., Meisinger, C., Döring, A., Altmaier, E., Belcredi, P., Gieger, C., ... Illig, T. (2010). Metabolic footprint of diabetes: A multiplatform metabolomics study in an epidemiological setting. PLoS One, 5(11), [e13953]. https://doi.org/10.1371/journal.pone.0013953

Metabolic footprint of diabetes : A multiplatform metabolomics study in an epidemiological setting. / Suhre, Karsten; Meisinger, Christa; Döring, Angela; Altmaier, Elisabeth; Belcredi, Petra; Gieger, Christian; Chang, David; Milburn, Michael V.; Gall, Walter E.; Weinberger, Klaus M.; Mewes, Hans Werner; Angelis, Martin Hrabé; Wichmann, H. Erich; Kronenberg, Florian; Adamski, Jerzy; Illig, Thomas.

In: PLoS One, Vol. 5, No. 11, e13953, 2010.

Research output: Contribution to journalArticle

Suhre, K, Meisinger, C, Döring, A, Altmaier, E, Belcredi, P, Gieger, C, Chang, D, Milburn, MV, Gall, WE, Weinberger, KM, Mewes, HW, Angelis, MH, Wichmann, HE, Kronenberg, F, Adamski, J & Illig, T 2010, 'Metabolic footprint of diabetes: A multiplatform metabolomics study in an epidemiological setting', PLoS One, vol. 5, no. 11, e13953. https://doi.org/10.1371/journal.pone.0013953
Suhre, Karsten ; Meisinger, Christa ; Döring, Angela ; Altmaier, Elisabeth ; Belcredi, Petra ; Gieger, Christian ; Chang, David ; Milburn, Michael V. ; Gall, Walter E. ; Weinberger, Klaus M. ; Mewes, Hans Werner ; Angelis, Martin Hrabé ; Wichmann, H. Erich ; Kronenberg, Florian ; Adamski, Jerzy ; Illig, Thomas. / Metabolic footprint of diabetes : A multiplatform metabolomics study in an epidemiological setting. In: PLoS One. 2010 ; Vol. 5, No. 11.
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