The major cell surface protein of a strain of human fetal lung fibroblasts is a 220,000 dalton glycoprotein termed fibronectin. Fibronectin is released from fibroblasts into the culture medium with a half-time of about 25 hours. The release occurs with an initial (0-3 hours) rapid phase followed by a second (3-48 hours) slower phase. Release of fibronectin occurs in a stoichiometric fashion. All of the fibronectin released from the cells is quantitatively recovered from the culture media as a similar sized soluble glycoprotein. Thus, release of fibronectin from normal human fibroblasts results from mechanisms other than extensive degradation of the protein.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 7 Nov 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology