Metabolic fate of the major cell surface protein of normal human fibroblasts

Bruce J. Baum, John A. McDonald, Ronald G. Crystal

Research output: Contribution to journalArticle

43 Citations (Scopus)


The major cell surface protein of a strain of human fetal lung fibroblasts is a 220,000 dalton glycoprotein termed fibronectin. Fibronectin is released from fibroblasts into the culture medium with a half-time of about 25 hours. The release occurs with an initial (0-3 hours) rapid phase followed by a second (3-48 hours) slower phase. Release of fibronectin occurs in a stoichiometric fashion. All of the fibronectin released from the cells is quantitatively recovered from the culture media as a similar sized soluble glycoprotein. Thus, release of fibronectin from normal human fibroblasts results from mechanisms other than extensive degradation of the protein.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 7 Nov 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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