Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay

The effect of circadian time, age and genetic background

L. Lin, J. Wisor, T. Shiba, Shahrad Taheri, K. Yanai, S. Wurts, X. Lin, M. Vitaterna, J. Takahashi, T. W. Lovenberg, M. Koehl, G. Uhl, S. Nishino, E. Mignot

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The hypocretins (1 and 2) have emerged as key regulators of sleep and wakefulness. We developed a high-throughput enzyme immunoassay (EIA) to measure total brain hypocretin levels from large numbers of mice. Hypocretin levels were not altered by circadian time or age. However, significant differences in one or both hypocretin peptides were observed between different mouse strains. We studied hypocretin levels in knockout and transgenic mouse models with obesity, circadian gene mutations or monoaminergic defects. Compared to controls, only histamine receptor knockouts had lower hypocretin levels. This was most pronounced in H1 receptor knockouts suggesting the existence of a positive feedback loop between hypocretin and histaminergic neurons.

Original languageEnglish
Pages (from-to)2203-2211
Number of pages9
JournalPeptides
Volume23
Issue number12
DOIs
Publication statusPublished - 1 Dec 2002
Externally publishedYes

Fingerprint

Immunoenzyme Techniques
Brain
Enzymes
Histamine Receptors
Histamine H1 Receptors
Genetic Background
Orexins
Wakefulness
Knockout Mice
Transgenic Mice
Neurons
Sleep
Obesity
Genes
Throughput
Feedback
Defects
Peptides
Mutation

Keywords

  • Enzyme immunoassay
  • Histamine receptor
  • Hypocretin
  • Knockout
  • Narcolepsy
  • Orexin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay : The effect of circadian time, age and genetic background. / Lin, L.; Wisor, J.; Shiba, T.; Taheri, Shahrad; Yanai, K.; Wurts, S.; Lin, X.; Vitaterna, M.; Takahashi, J.; Lovenberg, T. W.; Koehl, M.; Uhl, G.; Nishino, S.; Mignot, E.

In: Peptides, Vol. 23, No. 12, 01.12.2002, p. 2203-2211.

Research output: Contribution to journalArticle

Lin, L, Wisor, J, Shiba, T, Taheri, S, Yanai, K, Wurts, S, Lin, X, Vitaterna, M, Takahashi, J, Lovenberg, TW, Koehl, M, Uhl, G, Nishino, S & Mignot, E 2002, 'Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay: The effect of circadian time, age and genetic background', Peptides, vol. 23, no. 12, pp. 2203-2211. https://doi.org/10.1016/S0196-9781(02)00251-6
Lin, L. ; Wisor, J. ; Shiba, T. ; Taheri, Shahrad ; Yanai, K. ; Wurts, S. ; Lin, X. ; Vitaterna, M. ; Takahashi, J. ; Lovenberg, T. W. ; Koehl, M. ; Uhl, G. ; Nishino, S. ; Mignot, E. / Measurement of hypocretin/orexin content in the mouse brain using an enzyme immunoassay : The effect of circadian time, age and genetic background. In: Peptides. 2002 ; Vol. 23, No. 12. pp. 2203-2211.
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