Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration

Haijun Zhang, Jing Yang, Matthew S. Walters, Michelle R. Staudt, Yael Strulovici-Barel, Jacqueline Salit, Jason G. Mezey, Philip L. Leopold, Ronald Crystal

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Due to high levels of expression in aggressive tumors, high mobility group AThook 1 (HMGA1) has recently attracted attention as a potential anti-tumor target. However, HMGA1 is also expressed in normal somatic progenitor cells, raising the question: how might systemic anti-HMGA1 therapies affect the structure and function of normal tissue differentiation? In the present study, RNA sequencing data demonstrated HMGA1 is highly expressed in human airway basal stem/progenitor cells (BC), but decreases with BC differentiation in air-liquid interface cultures (ALI). BC collected from nonsmokers, healthy smokers, and smokers with chronic obstructive pulmonary disease (COPD) displayed a range of HMGA1 expression levels. Low initial expression levels of HMGA1 in BC were associated with decreased ability to maintain a differentiated ALI epithelium. HMGA1 down-regulation in BC diminished BC proliferation, suppressed gene expression related to normal proliferation and differentiation, decreased airway epithelial resistance, suppressed junctional and cell polarity gene expression, and delayed wound closure of airway epithelium following injury. Furthermore, silencing of HMGA1 in airway BC in ALI increased the expression of genes associated with airway remodeling in COPD including squamous, epithelialmesenchymal transition (EMT), and inflammatory genes. Together, the data suggests HMGA1 plays a central role in normal airway differentiation, and thus caution should be used to monitor airway epithelial structure and function in the context of systemic HMGA1-targeted therapies.

Original languageEnglish
Pages (from-to)14324-14337
Number of pages14
JournalOncotarget
Volume9
Issue number18
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Fingerprint

Regeneration
Stem Cells
Air
Gene Expression
Chronic Obstructive Pulmonary Disease
Wounds and Injuries
Epithelium
RNA Sequence Analysis
Airway Remodeling
Cell Polarity
Airway Resistance
Neoplasms
Down-Regulation
Therapeutics
Genes

Keywords

  • COPD
  • Differentiation
  • HMGA1
  • Regeneration
  • Wound repair

ASJC Scopus subject areas

  • Oncology

Cite this

Zhang, H., Yang, J., Walters, M. S., Staudt, M. R., Strulovici-Barel, Y., Salit, J., ... Crystal, R. (2018). Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. Oncotarget, 9(18), 14324-14337. https://doi.org/10.18632/oncotarget.24511

Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. / Zhang, Haijun; Yang, Jing; Walters, Matthew S.; Staudt, Michelle R.; Strulovici-Barel, Yael; Salit, Jacqueline; Mezey, Jason G.; Leopold, Philip L.; Crystal, Ronald.

In: Oncotarget, Vol. 9, No. 18, 01.01.2018, p. 14324-14337.

Research output: Contribution to journalArticle

Zhang, H, Yang, J, Walters, MS, Staudt, MR, Strulovici-Barel, Y, Salit, J, Mezey, JG, Leopold, PL & Crystal, R 2018, 'Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration', Oncotarget, vol. 9, no. 18, pp. 14324-14337. https://doi.org/10.18632/oncotarget.24511
Zhang H, Yang J, Walters MS, Staudt MR, Strulovici-Barel Y, Salit J et al. Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. Oncotarget. 2018 Jan 1;9(18):14324-14337. https://doi.org/10.18632/oncotarget.24511
Zhang, Haijun ; Yang, Jing ; Walters, Matthew S. ; Staudt, Michelle R. ; Strulovici-Barel, Yael ; Salit, Jacqueline ; Mezey, Jason G. ; Leopold, Philip L. ; Crystal, Ronald. / Mandatory role of HMGA1 in human airway epithelial normal differentiation and post-injury regeneration. In: Oncotarget. 2018 ; Vol. 9, No. 18. pp. 14324-14337.
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