Lymphocyte intracellular free calcium concentration is increased in preeclampsia

Minoru Hojo, Manikkam Suthanthiran, Geraldine Helseth, Phyllis August

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

OBJECTIVES: We tested 2 hypotheses: (1) Preeclampsia is characterized by an increase in intracellular free calcium concentration in lymphocytes. (2) Levels of intracellular free calcium are influenced by the calcium concentration in the extracellular milieu or by parathyroid hormone. STUDY DESIGN: Intracellular free calcium concentrations were measured in 4 groups of women: nonpregnant women (n = 25), normotensive pregnant women (n = 30), pregnant women with chronic hypertension (n = 15), and women with preeclampsia (n = 15). Intracellular free calcium concentration was measured in the basal state, at varying extracellular calcium ion concentrations, and in the presence of exogenous parathyroid hormone. RESULTS: Women with preeclampsia had the highest basal lymphocyte intracellular free calcium concentration (121±7 nmol/L, mean±SEM) compared with normotensive pregnant women during the third trimester (94±3 nmol/L, P < .001) and pregnant women in the third trimester with chronic hypertension (100±3 nmol/L, P < .01). During the third trimester normotensive women and women with chronic hypertension had significantly higher basal intracellular free calcium concentrations than were found in women during the first trimester. Exposure of lymphocytes to an extracellular milieu of low calcium concentration resulted in an increase in intracellular free calcium concentration, incubation with parathyroid hormone had no effect on intracellular free calcium concentration. CONCLUSIONS: Lymphocyte intracellular free calcium concentration is increased in preeclampsia and not in chronic hypertensive pregnancy and is greater during the third trimester than during the first trimester. Extracellular calcium depletion increases lymphocyte intracellular free calcium concentration. These data support the idea that a calcium deficit leading to an increased intracellular free calcium concentration during late pregnancy contributes to the pathogenesis of preeclampsia.

Original languageEnglish
Pages (from-to)1209-1214
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume180
Issue number5
DOIs
Publication statusPublished - 1 Jan 1999
Externally publishedYes

Fingerprint

Pre-Eclampsia
Lymphocytes
Calcium
Third Pregnancy Trimester
Pregnant Women
Parathyroid Hormone
First Pregnancy Trimester
Hypertension
Lymphocyte Depletion
Pregnancy

Keywords

  • Hypertension
  • Intracellular calcium
  • Preeclampsia

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Lymphocyte intracellular free calcium concentration is increased in preeclampsia. / Hojo, Minoru; Suthanthiran, Manikkam; Helseth, Geraldine; August, Phyllis.

In: American Journal of Obstetrics and Gynecology, Vol. 180, No. 5, 01.01.1999, p. 1209-1214.

Research output: Contribution to journalArticle

Hojo, Minoru ; Suthanthiran, Manikkam ; Helseth, Geraldine ; August, Phyllis. / Lymphocyte intracellular free calcium concentration is increased in preeclampsia. In: American Journal of Obstetrics and Gynecology. 1999 ; Vol. 180, No. 5. pp. 1209-1214.
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AU - Hojo, Minoru

AU - Suthanthiran, Manikkam

AU - Helseth, Geraldine

AU - August, Phyllis

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N2 - OBJECTIVES: We tested 2 hypotheses: (1) Preeclampsia is characterized by an increase in intracellular free calcium concentration in lymphocytes. (2) Levels of intracellular free calcium are influenced by the calcium concentration in the extracellular milieu or by parathyroid hormone. STUDY DESIGN: Intracellular free calcium concentrations were measured in 4 groups of women: nonpregnant women (n = 25), normotensive pregnant women (n = 30), pregnant women with chronic hypertension (n = 15), and women with preeclampsia (n = 15). Intracellular free calcium concentration was measured in the basal state, at varying extracellular calcium ion concentrations, and in the presence of exogenous parathyroid hormone. RESULTS: Women with preeclampsia had the highest basal lymphocyte intracellular free calcium concentration (121±7 nmol/L, mean±SEM) compared with normotensive pregnant women during the third trimester (94±3 nmol/L, P < .001) and pregnant women in the third trimester with chronic hypertension (100±3 nmol/L, P < .01). During the third trimester normotensive women and women with chronic hypertension had significantly higher basal intracellular free calcium concentrations than were found in women during the first trimester. Exposure of lymphocytes to an extracellular milieu of low calcium concentration resulted in an increase in intracellular free calcium concentration, incubation with parathyroid hormone had no effect on intracellular free calcium concentration. CONCLUSIONS: Lymphocyte intracellular free calcium concentration is increased in preeclampsia and not in chronic hypertensive pregnancy and is greater during the third trimester than during the first trimester. Extracellular calcium depletion increases lymphocyte intracellular free calcium concentration. These data support the idea that a calcium deficit leading to an increased intracellular free calcium concentration during late pregnancy contributes to the pathogenesis of preeclampsia.

AB - OBJECTIVES: We tested 2 hypotheses: (1) Preeclampsia is characterized by an increase in intracellular free calcium concentration in lymphocytes. (2) Levels of intracellular free calcium are influenced by the calcium concentration in the extracellular milieu or by parathyroid hormone. STUDY DESIGN: Intracellular free calcium concentrations were measured in 4 groups of women: nonpregnant women (n = 25), normotensive pregnant women (n = 30), pregnant women with chronic hypertension (n = 15), and women with preeclampsia (n = 15). Intracellular free calcium concentration was measured in the basal state, at varying extracellular calcium ion concentrations, and in the presence of exogenous parathyroid hormone. RESULTS: Women with preeclampsia had the highest basal lymphocyte intracellular free calcium concentration (121±7 nmol/L, mean±SEM) compared with normotensive pregnant women during the third trimester (94±3 nmol/L, P < .001) and pregnant women in the third trimester with chronic hypertension (100±3 nmol/L, P < .01). During the third trimester normotensive women and women with chronic hypertension had significantly higher basal intracellular free calcium concentrations than were found in women during the first trimester. Exposure of lymphocytes to an extracellular milieu of low calcium concentration resulted in an increase in intracellular free calcium concentration, incubation with parathyroid hormone had no effect on intracellular free calcium concentration. CONCLUSIONS: Lymphocyte intracellular free calcium concentration is increased in preeclampsia and not in chronic hypertensive pregnancy and is greater during the third trimester than during the first trimester. Extracellular calcium depletion increases lymphocyte intracellular free calcium concentration. These data support the idea that a calcium deficit leading to an increased intracellular free calcium concentration during late pregnancy contributes to the pathogenesis of preeclampsia.

KW - Hypertension

KW - Intracellular calcium

KW - Preeclampsia

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