Lung effector memory and activated CD4+ T cells display enhanced proliferation in surfactant protein A-deficient mice during allergen-mediated inflammation

Amy M. Pastva, Sambuddho Mukherjee, Charles Giamberardino, Bethany Hsia, Bernice Lo, Gregory D. Sempowski, Jo Rae Wright

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Although many studies have shown that pulmonary surfactant protein (SP)-A functions in innate immunity, fewer studies have addressed its role in adaptive immunity and allergic hypersensitivity. We hypothesized that SP-A modulates the phenotype and prevalence of dendritic cells (DCs) and CD4+ T cells to inhibit Th2-associated inflammatory indices associated with allergen-induced inflammation. In an OVA model of allergic hypersensitivity, SP-A-/- mice had greater eosinophilia, Th2-associated cytokine levels, and IgE levels compared with wild-type counterparts. Although both OVA-exposed groups had similar proportions of CD86+ DCs and Foxp3+ T regulatory cells, the SP-A-/- mice had elevated proportions of CD4+ activated and effector memory T cells in their lungs compared with wild-type mice. Ex vivo recall stimulation of CD4+ T cell pools demonstrated that cells from the SP-A-/- OVA mice had the greatest proliferative and IL-4 - producing capacity, and this capability was attenuated with exogenous SP-A treatment. Additionally, tracking proliferation in vivo demonstrated that CD4+ activated and effector memory T cells expanded to the greatest extent in the lungs of SP-A-/- OVA mice. Taken together, our data suggested that SP-A influences the prevalence, types, and functions of CD4 + T cells in the lungs during allergic inflammation and that SP deficiency modifies the severity of inflammation in allergic hypersensitivity conditions like asthma.

Original languageEnglish
Pages (from-to)2842-2849
Number of pages8
JournalJournal of Immunology
Volume186
Issue number5
DOIs
Publication statusPublished - 1 Mar 2011
Externally publishedYes

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Pulmonary Surfactant-Associated Protein A
Allergens
Inflammation
T-Lymphocytes
Lung
Pulmonary Surfactant-Associated Proteins
Hypersensitivity
Dendritic Cells
Protein Deficiency
Eosinophilia
Adaptive Immunity
Regulatory T-Lymphocytes
Innate Immunity
Surface-Active Agents
Interleukin-4
Immunoglobulin E
Asthma
Cytokines
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Lung effector memory and activated CD4+ T cells display enhanced proliferation in surfactant protein A-deficient mice during allergen-mediated inflammation. / Pastva, Amy M.; Mukherjee, Sambuddho; Giamberardino, Charles; Hsia, Bethany; Lo, Bernice; Sempowski, Gregory D.; Wright, Jo Rae.

In: Journal of Immunology, Vol. 186, No. 5, 01.03.2011, p. 2842-2849.

Research output: Contribution to journalArticle

Pastva, Amy M. ; Mukherjee, Sambuddho ; Giamberardino, Charles ; Hsia, Bethany ; Lo, Bernice ; Sempowski, Gregory D. ; Wright, Jo Rae. / Lung effector memory and activated CD4+ T cells display enhanced proliferation in surfactant protein A-deficient mice during allergen-mediated inflammation. In: Journal of Immunology. 2011 ; Vol. 186, No. 5. pp. 2842-2849.
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