Luminescent iridium(III)-containing block copolymers: Self-assembly into biotin-labeled micelles for biodetection assays

Kimberly L. Metera, Kevin D. Ha-nni, Gina Zhou, Manoj K. Nayak, Hassan S. Bazzi, David Juncker, Hanadi F. Sleiman

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Luminescent polymers containing Ir(ppy)2(bpy) PF6 complexes, biocompatible poly(ethylene glycol) (PEG) chains, and biotin moieties were synthesized via ring-opening metathesis polymerization (ROMP). Their self-assembly in water into micelles resulted in an increased quantum yield compared to open polymer chains in acetonitrile, which is likely due to core rigidity and desolvation. Streptavidin coated magnetic beads were employed to analyze the binding ability of these micelles. The positioning of the molecular recognition moiety biotin within the polymer chain had a very significant effect on the availability of biotin on the micelle surface and the ability of the micelles to bind to streptavidin. Simply attaching biotin to the end of the ROMP polymer yielded micelles in which the biotin units were shielded by the PEG chains, whereas the synthesis of a new ROMP monomer containing biotin at the end of the PEG chains resulted in improved surface availability of the biotin group. Preliminary experiments in which streptavidin was microcontact-printed onto functionalized glass coverslips also indicated specific binding between the micelles and streptavidin and further demonstrated the potential of these micelle systems to function as luminescent probes in solid-phase biodetection assays.

Original languageEnglish
Pages (from-to)954-959
Number of pages6
JournalACS Macro Letters
Volume1
Issue number8
DOIs
Publication statusPublished - 2012

Fingerprint

Iridium
Micelles
Biotin
Self assembly
Block copolymers
Assays
Streptavidin
Polyethylene glycols
Ring opening polymerization
Polymers
Availability
Molecular recognition
Quantum yield
Acetonitrile
Rigidity
Monomers
Glass
Water

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Inorganic Chemistry
  • Materials Chemistry

Cite this

Luminescent iridium(III)-containing block copolymers : Self-assembly into biotin-labeled micelles for biodetection assays. / Metera, Kimberly L.; Ha-nni, Kevin D.; Zhou, Gina; Nayak, Manoj K.; Bazzi, Hassan S.; Juncker, David; Sleiman, Hanadi F.

In: ACS Macro Letters, Vol. 1, No. 8, 2012, p. 954-959.

Research output: Contribution to journalArticle

Metera, Kimberly L. ; Ha-nni, Kevin D. ; Zhou, Gina ; Nayak, Manoj K. ; Bazzi, Hassan S. ; Juncker, David ; Sleiman, Hanadi F. / Luminescent iridium(III)-containing block copolymers : Self-assembly into biotin-labeled micelles for biodetection assays. In: ACS Macro Letters. 2012 ; Vol. 1, No. 8. pp. 954-959.
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