Lower prevalence of Chlamydia pneumoniae DNA compared with Chlamydia trachomatis DNA in synovial tissue of arthritis patients

H. Ralph Schumacher, Hervé C. Gérard, Thurayya Arayssi, José A. Pando, Patrick J. Branigan, Diego L. Saaibi, Alan P. Hudson

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Objective. To assess the presence of Chlamydia pneumoniae DNA in the joints of patients with reactive arthritis (ReA) anti other arthritides. Methods. DNA was prepared from synovial tissue (ST) and several synovial fluid (SF) samples from 188 patients with either ReA, undifferentiated oligoarthritis, or other forms of arthritis, and from 24 normal (non- arthritis) individuals. Preparations were screened using polymerase chain reaction (PCR) assays that independently targeted the C pneumoniae 16S ribosomal RNA and major outer membrane protein genes. Results. Twenty-seven of 212 ST samples (12.7%) were PCR positive for C pneumoniae DNA; 10 SF samples from these 27 patients were similarly positive. Among the PCR- positive patients, 3 had ReA, 2 had Reiter's syndrome, 7 had undifferentiated oligoarthritis, 4 had undifferentiated monarthritis, 6 had rheumatoid arthritis, and 5 had other forms of arthritis. No samples from normal control individuals were PCR positive. Conclusion. DNA of C pneumoniae is present in synovial specimens from some arthritis patients. The prevalence of this organism in the joints was lower than that of C trachomatis, and synovial presence of the organism was not associated with any distinct clinical syndrome. Widely disseminated nucleic acids such as those of C pneumoniae might have some role in the pathogenesis of several arthritides, since the organism was not found in the ST from normal control individuals.

Original languageEnglish
Pages (from-to)1889-1893
Number of pages5
JournalArthritis and Rheumatism
Volume42
Issue number9
DOIs
Publication statusPublished - Sep 1999
Externally publishedYes

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Chlamydophila pneumoniae
Chlamydia trachomatis
Arthritis
Reactive Arthritis
DNA
Pneumonia
Polymerase Chain Reaction
Synovial Fluid
Joints
16S Ribosomal RNA
Nucleic Acids
Rheumatoid Arthritis
Membrane Proteins
Genes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Lower prevalence of Chlamydia pneumoniae DNA compared with Chlamydia trachomatis DNA in synovial tissue of arthritis patients. / Schumacher, H. Ralph; Gérard, Hervé C.; Arayssi, Thurayya; Pando, José A.; Branigan, Patrick J.; Saaibi, Diego L.; Hudson, Alan P.

In: Arthritis and Rheumatism, Vol. 42, No. 9, 09.1999, p. 1889-1893.

Research output: Contribution to journalArticle

Schumacher, H. Ralph ; Gérard, Hervé C. ; Arayssi, Thurayya ; Pando, José A. ; Branigan, Patrick J. ; Saaibi, Diego L. ; Hudson, Alan P. / Lower prevalence of Chlamydia pneumoniae DNA compared with Chlamydia trachomatis DNA in synovial tissue of arthritis patients. In: Arthritis and Rheumatism. 1999 ; Vol. 42, No. 9. pp. 1889-1893.
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abstract = "Objective. To assess the presence of Chlamydia pneumoniae DNA in the joints of patients with reactive arthritis (ReA) anti other arthritides. Methods. DNA was prepared from synovial tissue (ST) and several synovial fluid (SF) samples from 188 patients with either ReA, undifferentiated oligoarthritis, or other forms of arthritis, and from 24 normal (non- arthritis) individuals. Preparations were screened using polymerase chain reaction (PCR) assays that independently targeted the C pneumoniae 16S ribosomal RNA and major outer membrane protein genes. Results. Twenty-seven of 212 ST samples (12.7{\%}) were PCR positive for C pneumoniae DNA; 10 SF samples from these 27 patients were similarly positive. Among the PCR- positive patients, 3 had ReA, 2 had Reiter's syndrome, 7 had undifferentiated oligoarthritis, 4 had undifferentiated monarthritis, 6 had rheumatoid arthritis, and 5 had other forms of arthritis. No samples from normal control individuals were PCR positive. Conclusion. DNA of C pneumoniae is present in synovial specimens from some arthritis patients. The prevalence of this organism in the joints was lower than that of C trachomatis, and synovial presence of the organism was not associated with any distinct clinical syndrome. Widely disseminated nucleic acids such as those of C pneumoniae might have some role in the pathogenesis of several arthritides, since the organism was not found in the ST from normal control individuals.",
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N2 - Objective. To assess the presence of Chlamydia pneumoniae DNA in the joints of patients with reactive arthritis (ReA) anti other arthritides. Methods. DNA was prepared from synovial tissue (ST) and several synovial fluid (SF) samples from 188 patients with either ReA, undifferentiated oligoarthritis, or other forms of arthritis, and from 24 normal (non- arthritis) individuals. Preparations were screened using polymerase chain reaction (PCR) assays that independently targeted the C pneumoniae 16S ribosomal RNA and major outer membrane protein genes. Results. Twenty-seven of 212 ST samples (12.7%) were PCR positive for C pneumoniae DNA; 10 SF samples from these 27 patients were similarly positive. Among the PCR- positive patients, 3 had ReA, 2 had Reiter's syndrome, 7 had undifferentiated oligoarthritis, 4 had undifferentiated monarthritis, 6 had rheumatoid arthritis, and 5 had other forms of arthritis. No samples from normal control individuals were PCR positive. Conclusion. DNA of C pneumoniae is present in synovial specimens from some arthritis patients. The prevalence of this organism in the joints was lower than that of C trachomatis, and synovial presence of the organism was not associated with any distinct clinical syndrome. Widely disseminated nucleic acids such as those of C pneumoniae might have some role in the pathogenesis of several arthritides, since the organism was not found in the ST from normal control individuals.

AB - Objective. To assess the presence of Chlamydia pneumoniae DNA in the joints of patients with reactive arthritis (ReA) anti other arthritides. Methods. DNA was prepared from synovial tissue (ST) and several synovial fluid (SF) samples from 188 patients with either ReA, undifferentiated oligoarthritis, or other forms of arthritis, and from 24 normal (non- arthritis) individuals. Preparations were screened using polymerase chain reaction (PCR) assays that independently targeted the C pneumoniae 16S ribosomal RNA and major outer membrane protein genes. Results. Twenty-seven of 212 ST samples (12.7%) were PCR positive for C pneumoniae DNA; 10 SF samples from these 27 patients were similarly positive. Among the PCR- positive patients, 3 had ReA, 2 had Reiter's syndrome, 7 had undifferentiated oligoarthritis, 4 had undifferentiated monarthritis, 6 had rheumatoid arthritis, and 5 had other forms of arthritis. No samples from normal control individuals were PCR positive. Conclusion. DNA of C pneumoniae is present in synovial specimens from some arthritis patients. The prevalence of this organism in the joints was lower than that of C trachomatis, and synovial presence of the organism was not associated with any distinct clinical syndrome. Widely disseminated nucleic acids such as those of C pneumoniae might have some role in the pathogenesis of several arthritides, since the organism was not found in the ST from normal control individuals.

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