Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients

S. C. Vasdev, L. Longerich, V. M. Prabhakaran, Christopher Triggle, M. H. Gault

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We assayed plasma Na,K-ATPase inhibitory activity due to total lipids and lipid fractions. The effect of dialysis on the Na,K-ATPase inhibitory activity was also studied. Plasma lipid extracts from 11 healthy volunteers and 9 dialysis-dependent patients (pre and post dialysis) were separated into neutral lipids and phospholipids. Further fractionation was by thin layer chromatography. These lipids fractions were analyzed for Na,K-ATPase inhibitory activity by displacement of [3H]-ouabain from hog brain Na,K-ATPase. Total inhibitory activity was significantly increased (p < 0.001) in the post-dialysis plasma compared to pre-dialysis plasma of the same patient group and to controls (482, 85 and 78 nmol/L respectively; means of the groups in digoxin equivalents). The major inhibitory activity was associated with non-esterified fatty acids with modest contributions from four other lipid fractions. Our results show that endogenous lipids are major plasma Na,K-ATPase inhibitors in vitro under these assay conditions.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalClinical Biochemistry
Volume22
Issue number4
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Dialysis
Lipids
Plasmas
Adenosine Triphosphatases
Thin layer chromatography
Digoxin
Ouabain
Fractionation
Thin Layer Chromatography
ATPase inhibitory protein
Assays
Brain
Phospholipids
Healthy Volunteers
Fatty Acids
Control Groups
sodium-translocating ATPase

Keywords

  • dialysis
  • digitalis-like
  • Na,K-ATPase inhibitors
  • non-esterified fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients. / Vasdev, S. C.; Longerich, L.; Prabhakaran, V. M.; Triggle, Christopher; Gault, M. H.

In: Clinical Biochemistry, Vol. 22, No. 4, 1989, p. 313-319.

Research output: Contribution to journalArticle

Vasdev, S. C. ; Longerich, L. ; Prabhakaran, V. M. ; Triggle, Christopher ; Gault, M. H. / Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients. In: Clinical Biochemistry. 1989 ; Vol. 22, No. 4. pp. 313-319.
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