Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients

S. C. Vasdev, L. Longerich, V. M. Prabhakaran, C. R. Triggle, M. H. Gault

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Abstract

We assayed plasma Na,K-ATPase inhibitory activity due to total lipids and lipid fractions. The effect of dialysis on the Na,K-ATPase inhibitory activity was also studied. Plasma lipid extracts from 11 healthy volunteers and 9 dialysis-dependent patients (pre and post dialysis) were separated into neutral lipids and phospholipids. Further fractionation was by thin layer chromatography. These lipids fractions were analyzed for Na,K-ATPase inhibitory activity by displacement of [3H]-ouabain from hog brain Na,K-ATPase. Total inhibitory activity was significantly increased (p < 0.001) in the post-dialysis plasma compared to pre-dialysis plasma of the same patient group and to controls (482, 85 and 78 nmol/L respectively; means of the groups in digoxin equivalents). The major inhibitory activity was associated with non-esterified fatty acids with modest contributions from four other lipid fractions. Our results show that endogenous lipids are major plasma Na,K-ATPase inhibitors in vitro under these assay conditions.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalClinical Biochemistry
Volume22
Issue number4
DOIs
Publication statusPublished - Aug 1989

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Keywords

  • Na,K-ATPase inhibitors
  • dialysis
  • digitalis-like
  • non-esterified fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry

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