Ligation of nanoparticle coated DNA cleaved with restriction enzymes

Joseph M. Kinsella, Margarita Shalaev, Albena Ivanisevic

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A λ phage DNA-based templates coated with CoFe2O4 nanoparticles, which can be cleaved by enzymes and subsequently ligated using a T4 ligase was demonstrated. The λ phage DNA used in this experiment has a theoretical stretched length of 16 4μm and has been shown to be capable of stretching to lengths longer than 20 μm using a molecular combing technique. The diameter of the structure was defined by the CoFe2O4 nanoparticles, which at 3.5 nm exceed the δ2 nm diameter of the DNA molecule. The restriction enzymes BamH1 and EcoR1, generate distinct size fragments due to their different sequence specificity. The restriction-digested samples were run on a 0.8% agarose gel to determine what affects the CoFe2O4 nanoparticle presence would have on the reaction. The BamH1 enzyme appears to produce all of the expected fragments in these samples, though the intensity of the samples appears to decrease with increasing concentrations of nanoparticles.

Original languageEnglish
Pages (from-to)3586-3588
Number of pages3
JournalChemistry of Materials
Volume19
Issue number15
DOIs
Publication statusPublished - 24 Jul 2007
Externally publishedYes

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DNA
Enzymes
Nanoparticles
Bacteriophages
Ligases
Sepharose
Stretching
Gels
Molecules
cobalt ferrite
Experiments

ASJC Scopus subject areas

  • Materials Chemistry
  • Materials Science(all)

Cite this

Ligation of nanoparticle coated DNA cleaved with restriction enzymes. / Kinsella, Joseph M.; Shalaev, Margarita; Ivanisevic, Albena.

In: Chemistry of Materials, Vol. 19, No. 15, 24.07.2007, p. 3586-3588.

Research output: Contribution to journalArticle

Kinsella, JM, Shalaev, M & Ivanisevic, A 2007, 'Ligation of nanoparticle coated DNA cleaved with restriction enzymes', Chemistry of Materials, vol. 19, no. 15, pp. 3586-3588. https://doi.org/10.1021/cm070850e
Kinsella, Joseph M. ; Shalaev, Margarita ; Ivanisevic, Albena. / Ligation of nanoparticle coated DNA cleaved with restriction enzymes. In: Chemistry of Materials. 2007 ; Vol. 19, No. 15. pp. 3586-3588.
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