Ligand profiling and identification technology for searching bioactive ligands

Moon Chang Baek, Sun Jin Kim, Kyungmoo Yea, Youndong Kim, Byung Dae Lee, Jaeyoon Kim, Hae Jeong Lee, Mee Hee Kang, Sunkyu Choi, Jong In Kim, Taehoon G. Lee, Pann Ghill Suh, Sung Ho Ryu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.

Original languageEnglish
Pages (from-to)1741-1749
Number of pages9
JournalProteomics
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Mar 2006
Externally publishedYes

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Keywords

  • Identification
  • Ligand
  • Peptidome
  • Profiling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Baek, M. C., Kim, S. J., Yea, K., Kim, Y., Lee, B. D., Kim, J., Lee, H. J., Kang, M. H., Choi, S., Kim, J. I., Lee, T. G., Suh, P. G., & Ryu, S. H. (2006). Ligand profiling and identification technology for searching bioactive ligands. Proteomics, 6(6), 1741-1749. https://doi.org/10.1002/pmic.200500511