Lentinula edodes (shiitake mushroom): An assessment of in vitro anti-atherosclerotic bio-functionality

Azizur Rahaman, Noorlidah Abdullah, Norhaniza Aminudin

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1 Citation (Scopus)


Mushrooms have been highly regarded as possessing enormous nutritive and medicinal values. In the present study, we evaluated the anti-oxidative and anti-atherosclerotic potential of shiitake mushroom (Lentinula edodes) using its solvent-solvent partitioned fractions that consisted of methanol:dichloromethane (M:DCM), hexane (HEX), dichloromethane (DCM), ethyl acetate (EA) and aqueous residue (AQ). The hexane fraction (1mg/mL) mostly scavenged (67.38%, IC50 0.55mg/mL) the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical, contained the highest reducing capacity (60.16mg gallic acid equivalents/g fraction), and most potently inhibited lipid peroxidation (67.07%), low density lipo-protein oxidation and the activity of 3-hydroxy 3-methyl glutaryl co-enzyme A reductase (HMGR). GC-MS analyses of the hexane fraction identified α-tocopherol (vitamin E), oleic acid, linoleic acid, ergosterol and butyric acid as the bio-functional components present in L. edodes. Our findings suggest that L. edodes possesses anti-atherosclerotic bio-functionality that can be applied as functional food-based therapeutics against cardiovascular diseases.

Original languageEnglish
JournalSaudi Journal of Biological Sciences
Publication statusAccepted/In press - 1 Jan 2016



  • Cardiovascular disease
  • Functional food
  • HMG Co-A reductase
  • Lipid peroxidation
  • Low density lipoprotein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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