Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MSn)

Hugues Preud'Homme, Johann Far, Sandra Gil-Casal, Ryszard Lobinski

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(iv)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (<1 ppm) and fragmentation (MS 2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.

Original languageEnglish
Pages (from-to)422-432
Number of pages11
JournalMetallomics
Volume4
Issue number5
DOIs
Publication statusPublished - 1 May 2012
Externally publishedYes

Fingerprint

Electrospray ionization
Electrospray Ionization Mass Spectrometry
Liquid chromatography
Selenium
Inductively coupled plasma
Reverse-Phase Chromatography
Metabolites
Mass spectrometry
Selenious Acid
Oligopeptides
Fractionation
Organic compounds
Yeast
Amino acids
Yeasts
Amino Acids

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics

Cite this

@article{b5ff5705d9c048ea837ba21ac039941c,
title = "Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MSn)",
abstract = "A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(iv)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (<1 ppm) and fragmentation (MS 2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.",
author = "Hugues Preud'Homme and Johann Far and Sandra Gil-Casal and Ryszard Lobinski",
year = "2012",
month = "5",
day = "1",
doi = "10.1039/c2mt00172a",
language = "English",
volume = "4",
pages = "422--432",
journal = "Metallomics",
issn = "1756-5901",
publisher = "Royal Society of Chemistry",
number = "5",

}

TY - JOUR

T1 - Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MSn)

AU - Preud'Homme, Hugues

AU - Far, Johann

AU - Gil-Casal, Sandra

AU - Lobinski, Ryszard

PY - 2012/5/1

Y1 - 2012/5/1

N2 - A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(iv)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (<1 ppm) and fragmentation (MS 2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.

AB - A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(iv)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (<1 ppm) and fragmentation (MS 2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.

UR - http://www.scopus.com/inward/record.url?scp=84860778883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860778883&partnerID=8YFLogxK

U2 - 10.1039/c2mt00172a

DO - 10.1039/c2mt00172a

M3 - Article

VL - 4

SP - 422

EP - 432

JO - Metallomics

JF - Metallomics

SN - 1756-5901

IS - 5

ER -