Recherche d'association entre le polymorphisme -308 G/A du gène du facteur nécrosant des tumeurs de type alpha (TNF-α) et la sévérité de la fibrose hépatique chez des patients Tunisiens infectés par le vi

Translated title of the contribution: Lack of effect of tumor necrosis factor-alpha -308 G/A polymorphism on severity of liver fibrosis in Tunisian hepatitis C virus (HCV)-infected patients

N. Bouzgarrou, E. Hassen, S. Gabbouj, E. Schvoerer, N. Ben Mami, H. Triki, Lotfi Chouchane

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: Tumor necrosis factor alpha (TNF-α) plays a key role in the immune response. An elevated plasma level of TNF-α was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-α expression. In this study, we aimed to evaluate the impact of TNF-α -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. Methods: TNF-α -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). Results: The genotype distribution of the TNF-α -308 G/A polymorphism among the HCV-infected patients was as follows: GG: 67.9%, GA: 32.1%, AA: 0%. With regard to fibrosis score, no significant differences in TNF-α genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15). Conclusion: No significant association between TNF-α -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.

Original languageFrench
Pages (from-to)297-304
Number of pages8
JournalGastroenterologie Clinique et Biologique
Volume34
Issue number4-5
DOIs
Publication statusPublished - Apr 2010

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Hepacivirus
Liver Cirrhosis
Tumor Necrosis Factor-alpha
Fibrosis
Genotype
Chronic Hepatitis C
Genetic Promoter Regions
Restriction Fragment Length Polymorphisms
Single Nucleotide Polymorphism
Polymerase Chain Reaction
Liver
Wounds and Injuries

ASJC Scopus subject areas

  • Medicine(all)
  • Gastroenterology

Cite this

@article{39d71106212748febba6a90e5720b6bd,
title = "Recherche d'association entre le polymorphisme -308 G/A du g{\`e}ne du facteur n{\'e}crosant des tumeurs de type alpha (TNF-α) et la s{\'e}v{\'e}rit{\'e} de la fibrose h{\'e}patique chez des patients Tunisiens infect{\'e}s par le vi",
abstract = "Objectives: Tumor necrosis factor alpha (TNF-α) plays a key role in the immune response. An elevated plasma level of TNF-α was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-α expression. In this study, we aimed to evaluate the impact of TNF-α -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. Methods: TNF-α -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). Results: The genotype distribution of the TNF-α -308 G/A polymorphism among the HCV-infected patients was as follows: GG: 67.9{\%}, GA: 32.1{\%}, AA: 0{\%}. With regard to fibrosis score, no significant differences in TNF-α genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15). Conclusion: No significant association between TNF-α -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.",
author = "N. Bouzgarrou and E. Hassen and S. Gabbouj and E. Schvoerer and {Ben Mami}, N. and H. Triki and Lotfi Chouchane",
year = "2010",
month = "4",
doi = "10.1016/j.gcb.2010.03.008",
language = "French",
volume = "34",
pages = "297--304",
journal = "Clinics and Research in Hepatology and Gastroenterology",
issn = "2210-7401",
publisher = "Elsevier Masson",
number = "4-5",

}

TY - JOUR

T1 - Recherche d'association entre le polymorphisme -308 G/A du gène du facteur nécrosant des tumeurs de type alpha (TNF-α) et la sévérité de la fibrose hépatique chez des patients Tunisiens infectés par le vi

AU - Bouzgarrou, N.

AU - Hassen, E.

AU - Gabbouj, S.

AU - Schvoerer, E.

AU - Ben Mami, N.

AU - Triki, H.

AU - Chouchane, Lotfi

PY - 2010/4

Y1 - 2010/4

N2 - Objectives: Tumor necrosis factor alpha (TNF-α) plays a key role in the immune response. An elevated plasma level of TNF-α was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-α expression. In this study, we aimed to evaluate the impact of TNF-α -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. Methods: TNF-α -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). Results: The genotype distribution of the TNF-α -308 G/A polymorphism among the HCV-infected patients was as follows: GG: 67.9%, GA: 32.1%, AA: 0%. With regard to fibrosis score, no significant differences in TNF-α genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15). Conclusion: No significant association between TNF-α -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.

AB - Objectives: Tumor necrosis factor alpha (TNF-α) plays a key role in the immune response. An elevated plasma level of TNF-α was repeatedly observed in patients with active liver injury or cirrhosis regardless of the aetiology. The G/A transition at position -308 in the promoter region have been shown to influence TNF-α expression. In this study, we aimed to evaluate the impact of TNF-α -308 G/A functional polymorphism on fibrosis severity in Tunisian Hepatitis C Virus (HCV)-infected patients. Methods: TNF-α -308 G/A polymorphism was evaluated by polymerase chain reaction (PCR) amplification followed by Restriction Fragment Length Polymorphism (RFLP) method in 53 chronic hepatitis C patients. Single-nucleotide polymorphism (SNP) frequencies were compared with regard to liver fibrosis severity as assessed by the METAVIR scoring system (F1-F2; n=22 versus F3-F4; n=31). Results: The genotype distribution of the TNF-α -308 G/A polymorphism among the HCV-infected patients was as follows: GG: 67.9%, GA: 32.1%, AA: 0%. With regard to fibrosis score, no significant differences in TNF-α genotype distribution were observed between F1-F2 and F3-F4 patients (p=0.15). Conclusion: No significant association between TNF-α -308 polymorphism and and the severity of liver fibrosis was found in our Tunisian cohort.

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