Kinetics of TCR use in response to repeated epitope-specific immunization

V. Monsurrò, M. B. Nielsen, A. Perez-Diez, M. E. Dudley, E. Wang, S. A. Rosenberg, F. M. Marincola

Research output: Contribution to journalArticle

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Abstract

Selection of T cell-directed immunization strategies is based extensively on discordant information derived from preclinical models. We characterized the kinetics of T cell selection in response to repeated antigenic challenge. By enumerating with epitope/HLA tetrameric complexes (tHLA) vaccine-elicited T cell precursor frequencies (Tc-pf) in melanoma patients exposed to the modified gp100 epitope gp100:209-217 (g209-2M) we observed in most patients that the Tc-pf increased with number of immunizations. One patient's kinetics were further characterized. Dissociation kinetics of g209-2M/tHLA suggested enrichment of T cell effector populations expressing TCR with progressively higher affinity. Furthermore, vaccine-elicited T cells maintained the ability to express IFN-γ ex vivo and proliferate in vitro. Thus, repeated exposure to immunogenic peptides benefited immune competence. These results provide a rationale for immunization strategies.

Original languageEnglish
Pages (from-to)5817-5825
Number of pages9
JournalJournal of Immunology
Volume166
Issue number9
Publication statusPublished - 1 May 2001
Externally publishedYes

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Epitopes
Immunization
T-Lymphoid Precursor Cells
T-Lymphocytes
Vaccines
Aptitude
Mental Competency
Melanoma
Peptides
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Monsurrò, V., Nielsen, M. B., Perez-Diez, A., Dudley, M. E., Wang, E., Rosenberg, S. A., & Marincola, F. M. (2001). Kinetics of TCR use in response to repeated epitope-specific immunization. Journal of Immunology, 166(9), 5817-5825.

Kinetics of TCR use in response to repeated epitope-specific immunization. / Monsurrò, V.; Nielsen, M. B.; Perez-Diez, A.; Dudley, M. E.; Wang, E.; Rosenberg, S. A.; Marincola, F. M.

In: Journal of Immunology, Vol. 166, No. 9, 01.05.2001, p. 5817-5825.

Research output: Contribution to journalArticle

Monsurrò, V, Nielsen, MB, Perez-Diez, A, Dudley, ME, Wang, E, Rosenberg, SA & Marincola, FM 2001, 'Kinetics of TCR use in response to repeated epitope-specific immunization', Journal of Immunology, vol. 166, no. 9, pp. 5817-5825.
Monsurrò V, Nielsen MB, Perez-Diez A, Dudley ME, Wang E, Rosenberg SA et al. Kinetics of TCR use in response to repeated epitope-specific immunization. Journal of Immunology. 2001 May 1;166(9):5817-5825.
Monsurrò, V. ; Nielsen, M. B. ; Perez-Diez, A. ; Dudley, M. E. ; Wang, E. ; Rosenberg, S. A. ; Marincola, F. M. / Kinetics of TCR use in response to repeated epitope-specific immunization. In: Journal of Immunology. 2001 ; Vol. 166, No. 9. pp. 5817-5825.
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