Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques

Ussama M. Abdel-Motal, Jacqueline Gillis, Kelledy Manson, Michael Wyand, David Montefiori, Kelly Stefano-Cole, Ronald C. Montelaro, John D. Altman, R. Paul Johnson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The ability of memory T cells to mount a recall response plays a key role in the ability of vaccinated animals to contain viral challenge. In this study, we intensively monitored the expansion of SIV Gag-specific CD8+ T cells in peripheral blood and tissues of rhesus macaques vaccinated with the attenuated strain SIVmac239Δ3 and challenged with the pathogenic viruses SIVmac239 or SIVsmE660. Although all vaccinated animals were infected with challenge virus, peak levels of plasma viremia in vaccinees were decreased by 1.5 to 2 logs as compared with naive controls. Decreased levels of plasma viremia in vaccinated animals were evident as early as 7 days post-challenge, well before the expansion of SIV-specific CD8+ T cells. Expansion of SIV-specific CD8+ T cells was not observed in peripheral blood or tissues until at least 14 days after infection and did not occur in most animals until after the initial peak of viral replication. The observation that expansion of SIV-specific CD8+ T cells is delayed until 7 days or more after initial detection of viremia highlights fundamental limitations in the ability of lentivirus-specific CD8+ T cells to mediate protection against challenge.

Original languageEnglish
Pages (from-to)226-238
Number of pages13
JournalVirology
Volume333
Issue number2
DOIs
Publication statusPublished - 15 Mar 2005
Externally publishedYes

Fingerprint

Macaca
T-Lymphocytes
Viremia
Viruses
Lentivirus
Macaca mulatta
Infection

Keywords

  • AIDS vaccine
  • Cytotoxic T lymphocytes
  • Live attenuated SIV vaccine
  • Mucosal immunity
  • Neutralizing antibodies
  • Nonhuman primates
  • Protective immunity
  • Simian immunodeficiency virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Abdel-Motal, U. M., Gillis, J., Manson, K., Wyand, M., Montefiori, D., Stefano-Cole, K., ... Johnson, R. P. (2005). Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques. Virology, 333(2), 226-238. https://doi.org/10.1016/j.virol.2004.12.030

Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques. / Abdel-Motal, Ussama M.; Gillis, Jacqueline; Manson, Kelledy; Wyand, Michael; Montefiori, David; Stefano-Cole, Kelly; Montelaro, Ronald C.; Altman, John D.; Johnson, R. Paul.

In: Virology, Vol. 333, No. 2, 15.03.2005, p. 226-238.

Research output: Contribution to journalArticle

Abdel-Motal, UM, Gillis, J, Manson, K, Wyand, M, Montefiori, D, Stefano-Cole, K, Montelaro, RC, Altman, JD & Johnson, RP 2005, 'Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques', Virology, vol. 333, no. 2, pp. 226-238. https://doi.org/10.1016/j.virol.2004.12.030
Abdel-Motal, Ussama M. ; Gillis, Jacqueline ; Manson, Kelledy ; Wyand, Michael ; Montefiori, David ; Stefano-Cole, Kelly ; Montelaro, Ronald C. ; Altman, John D. ; Johnson, R. Paul. / Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques. In: Virology. 2005 ; Vol. 333, No. 2. pp. 226-238.
@article{abd60b615e904f2888d98486d2a6a5dd,
title = "Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques",
abstract = "The ability of memory T cells to mount a recall response plays a key role in the ability of vaccinated animals to contain viral challenge. In this study, we intensively monitored the expansion of SIV Gag-specific CD8+ T cells in peripheral blood and tissues of rhesus macaques vaccinated with the attenuated strain SIVmac239Δ3 and challenged with the pathogenic viruses SIVmac239 or SIVsmE660. Although all vaccinated animals were infected with challenge virus, peak levels of plasma viremia in vaccinees were decreased by 1.5 to 2 logs as compared with naive controls. Decreased levels of plasma viremia in vaccinated animals were evident as early as 7 days post-challenge, well before the expansion of SIV-specific CD8+ T cells. Expansion of SIV-specific CD8+ T cells was not observed in peripheral blood or tissues until at least 14 days after infection and did not occur in most animals until after the initial peak of viral replication. The observation that expansion of SIV-specific CD8+ T cells is delayed until 7 days or more after initial detection of viremia highlights fundamental limitations in the ability of lentivirus-specific CD8+ T cells to mediate protection against challenge.",
keywords = "AIDS vaccine, Cytotoxic T lymphocytes, Live attenuated SIV vaccine, Mucosal immunity, Neutralizing antibodies, Nonhuman primates, Protective immunity, Simian immunodeficiency virus",
author = "Abdel-Motal, {Ussama M.} and Jacqueline Gillis and Kelledy Manson and Michael Wyand and David Montefiori and Kelly Stefano-Cole and Montelaro, {Ronald C.} and Altman, {John D.} and Johnson, {R. Paul}",
year = "2005",
month = "3",
day = "15",
doi = "10.1016/j.virol.2004.12.030",
language = "English",
volume = "333",
pages = "226--238",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Kinetics of expansion of SIV Gag-specific CD8+ T lymphocytes following challenge of vaccinated macaques

AU - Abdel-Motal, Ussama M.

AU - Gillis, Jacqueline

AU - Manson, Kelledy

AU - Wyand, Michael

AU - Montefiori, David

AU - Stefano-Cole, Kelly

AU - Montelaro, Ronald C.

AU - Altman, John D.

AU - Johnson, R. Paul

PY - 2005/3/15

Y1 - 2005/3/15

N2 - The ability of memory T cells to mount a recall response plays a key role in the ability of vaccinated animals to contain viral challenge. In this study, we intensively monitored the expansion of SIV Gag-specific CD8+ T cells in peripheral blood and tissues of rhesus macaques vaccinated with the attenuated strain SIVmac239Δ3 and challenged with the pathogenic viruses SIVmac239 or SIVsmE660. Although all vaccinated animals were infected with challenge virus, peak levels of plasma viremia in vaccinees were decreased by 1.5 to 2 logs as compared with naive controls. Decreased levels of plasma viremia in vaccinated animals were evident as early as 7 days post-challenge, well before the expansion of SIV-specific CD8+ T cells. Expansion of SIV-specific CD8+ T cells was not observed in peripheral blood or tissues until at least 14 days after infection and did not occur in most animals until after the initial peak of viral replication. The observation that expansion of SIV-specific CD8+ T cells is delayed until 7 days or more after initial detection of viremia highlights fundamental limitations in the ability of lentivirus-specific CD8+ T cells to mediate protection against challenge.

AB - The ability of memory T cells to mount a recall response plays a key role in the ability of vaccinated animals to contain viral challenge. In this study, we intensively monitored the expansion of SIV Gag-specific CD8+ T cells in peripheral blood and tissues of rhesus macaques vaccinated with the attenuated strain SIVmac239Δ3 and challenged with the pathogenic viruses SIVmac239 or SIVsmE660. Although all vaccinated animals were infected with challenge virus, peak levels of plasma viremia in vaccinees were decreased by 1.5 to 2 logs as compared with naive controls. Decreased levels of plasma viremia in vaccinated animals were evident as early as 7 days post-challenge, well before the expansion of SIV-specific CD8+ T cells. Expansion of SIV-specific CD8+ T cells was not observed in peripheral blood or tissues until at least 14 days after infection and did not occur in most animals until after the initial peak of viral replication. The observation that expansion of SIV-specific CD8+ T cells is delayed until 7 days or more after initial detection of viremia highlights fundamental limitations in the ability of lentivirus-specific CD8+ T cells to mediate protection against challenge.

KW - AIDS vaccine

KW - Cytotoxic T lymphocytes

KW - Live attenuated SIV vaccine

KW - Mucosal immunity

KW - Neutralizing antibodies

KW - Nonhuman primates

KW - Protective immunity

KW - Simian immunodeficiency virus

UR - http://www.scopus.com/inward/record.url?scp=13844294303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844294303&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2004.12.030

DO - 10.1016/j.virol.2004.12.030

M3 - Article

C2 - 15721357

AN - SCOPUS:13844294303

VL - 333

SP - 226

EP - 238

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -