Kinase-dependent regulation of inositol 1,4,5-trisphosphate-dependent Ca2+ release during oocyte maturation

Lu Sun, Shirley Haun, Richard C. Jones, Ricky D. Edmondson, Khaled Machaca

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Fertilization induces a species-specific Ca2+ transient with specialized spatial and temporal dynamics, which are essential to temporally encode egg activation events such as the block to polyspermy and resumption of meiosis. Eggs acquire the competence to produce the fertilization-specific Ca2+ transient during oocyte maturation, which encompasses dramatic potentiation of inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ release. Here we show that increased IP3 receptor (IP3R) sensitivity is initiated at the germinal vesicle breakdown stage of maturation, which correlates with maturation promoting factor (MPF) activation. Extensive phosphopeptide mapping of the IP3R resulted ∼70% coverage and identified three residues, Thr-931, Thr-1136, and Ser-114, which are specifically phosphorylated during maturation. Phospho-specific antibody analyses show that Thr-1136 phosphorylation requires MPF activation. Activation of either MPF or the mitogen-activated protein kinase cascade independently, functionally sensitizes IP3-dependent Ca2+ release. Collectively, these data argue that the kinase cascades driving meiotic maturation potentiates IP3-dependent Ca2+ release, possibly trough direct phosphorylation of the IP3R.

Original languageEnglish
Pages (from-to)20184-20196
Number of pages13
JournalJournal of Biological Chemistry
Volume284
Issue number30
DOIs
Publication statusPublished - 24 Jul 2009

Fingerprint

Maturation-Promoting Factor
Inositol 1,4,5-Trisphosphate Receptors
Inositol 1,4,5-Trisphosphate
Oocytes
Phosphotransferases
Chemical activation
Fertilization
Phosphorylation
Phospho-Specific Antibodies
Phosphopeptides
Meiosis
Mitogen-Activated Protein Kinases
Mental Competency
Eggs
Ovum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kinase-dependent regulation of inositol 1,4,5-trisphosphate-dependent Ca2+ release during oocyte maturation. / Sun, Lu; Haun, Shirley; Jones, Richard C.; Edmondson, Ricky D.; Machaca, Khaled.

In: Journal of Biological Chemistry, Vol. 284, No. 30, 24.07.2009, p. 20184-20196.

Research output: Contribution to journalArticle

Sun, Lu ; Haun, Shirley ; Jones, Richard C. ; Edmondson, Ricky D. ; Machaca, Khaled. / Kinase-dependent regulation of inositol 1,4,5-trisphosphate-dependent Ca2+ release during oocyte maturation. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 30. pp. 20184-20196.
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