KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma

Arcangela G. Manente, Giulia Pinton, Sara Zonca, Daniela Tavian, Tanwir Habib, Puthen V. Jithesh, Dean Fennell, Stefan Nilsson, Laura Moro

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). Materials & methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. Results & conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.

Original languageEnglish
Pages (from-to)1227-1238
Number of pages12
JournalEpigenomics
Volume8
Issue number9
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

Histone Demethylases
Mesothelioma
Epigenomics
Estrogen Receptors
Tumor Cell Line
Phenotype
Microarray Analysis
Computer Simulation
Histones
Methylation
Real-Time Polymerase Chain Reaction
Estrogens
Western Blotting
Gene Expression
Malignant Mesothelioma

Keywords

  • epigenetic
  • ERβ
  • H3K27me3
  • human malignant pleural mesothelioma
  • KDM6B

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma. / Manente, Arcangela G.; Pinton, Giulia; Zonca, Sara; Tavian, Daniela; Habib, Tanwir; Jithesh, Puthen V.; Fennell, Dean; Nilsson, Stefan; Moro, Laura.

In: Epigenomics, Vol. 8, No. 9, 01.09.2016, p. 1227-1238.

Research output: Contribution to journalArticle

Manente, Arcangela G. ; Pinton, Giulia ; Zonca, Sara ; Tavian, Daniela ; Habib, Tanwir ; Jithesh, Puthen V. ; Fennell, Dean ; Nilsson, Stefan ; Moro, Laura. / KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma. In: Epigenomics. 2016 ; Vol. 8, No. 9. pp. 1227-1238.
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AU - Habib, Tanwir

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AU - Fennell, Dean

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