JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium

Kazunori Gomi, Michelle R. Staudt, Jacqueline Salit, Robert J. Kaner, Jonna Heldrich, Allison M. Rogalski, Vanessa Arbelaez, Ronald G. Crystal, Matthew S. Walters

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Basal cells (BC) are the stem/progenitor cells of the human airway epithelium capable of differentiating into secretory and ciliated cells. Notch signaling activation increases BC differentiation into secretory cells, but the role of individual Notch ligands in regulating this process in the human airway epithelium is largely unknown. The objective of this study was to define the role of the Notch ligand JAG1 in regulating human BC differentiation. JAG1 over-expression in BC increased secretory cell differentiation, with no effect on ciliated cell differentiation. Conversely, knockdown of JAG1 decreased expression of secretory cell genes. These data demonstrate JAG1-mediated Notch signaling regulates differentiation of BC into secretory cells.

Original languageEnglish
Pages (from-to)454-463
Number of pages10
JournalStem Cell Reviews and Reports
Volume12
Issue number4
DOIs
Publication statusPublished - 1 Aug 2016

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Keywords

  • Basal stem/progenitor cells
  • Differentiation
  • Human airway epithelium
  • JAG1
  • Notch signaling

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Gomi, K., Staudt, M. R., Salit, J., Kaner, R. J., Heldrich, J., Rogalski, A. M., Arbelaez, V., Crystal, R. G., & Walters, M. S. (2016). JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium. Stem Cell Reviews and Reports, 12(4), 454-463. https://doi.org/10.1007/s12015-016-9656-6