Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library

I. M. Chumakov, I. Le Gall, A. Billault, P. Ougen, P. Soularue, S. Guillou, P. Rigault, H. Bui, M. F. De Tand, E. Barillot, H. Abderrahim, D. Cherif, R. Berger, D. Le Paslier, D. Cohen

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.

Original languageEnglish
Pages (from-to)222-225
Number of pages4
JournalNature Genetics
Volume1
Issue number3
Publication statusPublished - 1 Dec 1992
Externally publishedYes

Fingerprint

Yeast Artificial Chromosomes
Chromosomes, Human, Pair 21
Genomic Library
Human Genome
Clone Cells
Chromosomes
Polymerase Chain Reaction
Hybrid Cells
Human Chromosomes
In Situ Hybridization
Genome
Cell Line
sorbitan monolaurate

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Chumakov, I. M., Le Gall, I., Billault, A., Ougen, P., Soularue, P., Guillou, S., ... Cohen, D. (1992). Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library. Nature Genetics, 1(3), 222-225.

Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library. / Chumakov, I. M.; Le Gall, I.; Billault, A.; Ougen, P.; Soularue, P.; Guillou, S.; Rigault, P.; Bui, H.; De Tand, M. F.; Barillot, E.; Abderrahim, H.; Cherif, D.; Berger, R.; Le Paslier, D.; Cohen, D.

In: Nature Genetics, Vol. 1, No. 3, 01.12.1992, p. 222-225.

Research output: Contribution to journalArticle

Chumakov, IM, Le Gall, I, Billault, A, Ougen, P, Soularue, P, Guillou, S, Rigault, P, Bui, H, De Tand, MF, Barillot, E, Abderrahim, H, Cherif, D, Berger, R, Le Paslier, D & Cohen, D 1992, 'Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library', Nature Genetics, vol. 1, no. 3, pp. 222-225.
Chumakov IM, Le Gall I, Billault A, Ougen P, Soularue P, Guillou S et al. Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library. Nature Genetics. 1992 Dec 1;1(3):222-225.
Chumakov, I. M. ; Le Gall, I. ; Billault, A. ; Ougen, P. ; Soularue, P. ; Guillou, S. ; Rigault, P. ; Bui, H. ; De Tand, M. F. ; Barillot, E. ; Abderrahim, H. ; Cherif, D. ; Berger, R. ; Le Paslier, D. ; Cohen, D. / Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library. In: Nature Genetics. 1992 ; Vol. 1, No. 3. pp. 222-225.
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