Abstract
A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
| Original language | English |
|---|---|
| Pages (from-to) | 222-225 |
| Number of pages | 4 |
| Journal | Nature Genetics |
| Volume | 1 |
| Issue number | 3 |
| Publication status | Published - 1 Dec 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library. / Chumakov, I. M.; Le Gall, I.; Billault, A.; Ougen, P.; Soularue, P.; Guillou, S.; Rigault, P.; Bui, H.; De Tand, M. F.; Barillot, E.; Abderrahim, H.; Cherif, D.; Berger, R.; Le Paslier, D.; Cohen, D.
In: Nature Genetics, Vol. 1, No. 3, 01.12.1992, p. 222-225.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library
AU - Chumakov, I. M.
AU - Le Gall, I.
AU - Billault, A.
AU - Ougen, P.
AU - Soularue, P.
AU - Guillou, S.
AU - Rigault, P.
AU - Bui, H.
AU - De Tand, M. F.
AU - Barillot, E.
AU - Abderrahim, H.
AU - Cherif, D.
AU - Berger, R.
AU - Le Paslier, D.
AU - Cohen, D.
PY - 1992/12/1
Y1 - 1992/12/1
N2 - A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
AB - A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026878997&partnerID=8YFLogxK
M3 - Article
VL - 1
SP - 222
EP - 225
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 3
ER -