Isolation, characterization, and bioinformatic analysis of calmodulin-binding protein cmbB reveals a novel tandem IP22 repeat common to many Dictyostelium and Mimivirus proteins

Danton H. O'Day, Karsten Suhre, Michael A. Myre, Munmun Chatterjee-Chakraborty, Sara E. Chavez

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel calmodulin-binding protein cmbB from Dictyostelium discoideum is encoded in a single gene. Northern analysis reveals two cmbB transcripts first detectable at 4 h during multicellular development. Western blotting detects an ∼46.6 kDa protein. Sequence analysis and calmodulin-agarose binding studies identified a "classic" calcium-dependent calmodulin-binding domain (179IPKSLRSLFLGKGYNQPLEF198) but structural analyses suggest binding may not involve classic α-helical calmodulin-binding. The cmbB protein is comprised of tandem repeats of a newly identified IP22 motif ([I,L]Pxxhxxhxhxxxhxxxhxxxx; where h = any hydrophobic amino acid) that is highly conserved and a more precise representation of the FNIP repeat. At least eight Acanthamoeba polyphaga Mimivirus proteins and over 100 Dictyostelium proteins contain tandem arrays of the IP22 motif and its variants. cmbB also shares structural homology to YopM, from the plague bacterium Yersenia pestis.

Original languageEnglish
Pages (from-to)879-888
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number3
DOIs
Publication statusPublished - 4 Aug 2006
Externally publishedYes

Fingerprint

Mimiviridae
Calmodulin-Binding Proteins
Tandem Repeat Sequences
Dictyostelium
Bioinformatics
Computational Biology
Calmodulin
Proteins
Plague
Sepharose
Sequence Analysis
Bacteria
Genes
Western Blotting
Calcium
Amino Acids

Keywords

  • Calmodulin-binding protein
  • CaMBOT
  • Dictyostelium
  • FNIP repeat
  • Leucine rich repeat
  • Mimivirus
  • YopM

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation, characterization, and bioinformatic analysis of calmodulin-binding protein cmbB reveals a novel tandem IP22 repeat common to many Dictyostelium and Mimivirus proteins. / O'Day, Danton H.; Suhre, Karsten; Myre, Michael A.; Chatterjee-Chakraborty, Munmun; Chavez, Sara E.

In: Biochemical and Biophysical Research Communications, Vol. 346, No. 3, 04.08.2006, p. 879-888.

Research output: Contribution to journalArticle

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