Isolation and genomic characterization of the TUPLE1/HIRA gene of the pufferfish Fugu rubripes

Roser Llevadot, Xavier P. Estivill, Peter Scambler, Melanie Pritchard

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In an effort to obtain a small genomic construct for the generation of a HIRA transgenic mouse, we have isolated and sequenced the Fugu TUPLE1/HIRA gene. We have compared the gene organization and the proteins encoded in pufferfish and human and also searched for conserved DNA sequences that might be important in gene regulation. The pufferfish gene spans approx. 9 kb, which is approx. 11 times smaller than the human gene, owing to the reduced size of the introns. Like its human counterpart, it is organized into 25 exons. The majority of the splice sites are in identical positions to those found in the human gene, however, for three internal exons the positions of the splice sites are not directly comparable. The coding regions are almost identical in size and show a high degree of similarity, especially at the amino and carboxy termini. Comparisons of 5' and 3' sequences failed to detect similarities or sequences involved in regulation.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalGene
Volume208
Issue number2
DOIs
Publication statusPublished - 22 Feb 1998
Externally publishedYes

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Keywords

  • Comparative genetics
  • DiGeorge syndrome
  • Gene organization

ASJC Scopus subject areas

  • Genetics

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