Involvement of nitrosothiols, nitric oxide and voltage-gated K+ channels in photorelaxation of vascular smooth muscle

Fina Lovren, Chris R. Triggle

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effects of nitrosothiol depleting compounds (p- hydroxymercuribenzoate, iodacetamide and ethacrynic acid), a guanylyl cyclase inhibitor (1 H[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, ODQ) and nitric oxide (NO) scavenger agents (xanthine/xanthine oxidase and 2-(4-carboxyphenyl)- 4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide; carboxy-PTIO) on light- induced photorelaxation in rat thoracic aorta were investigated. Photorelaxation responses were decreased in the presence of nitrosothiol depleting compounds suggesting S-nitrosothiols as the tissue source of the NO, whereas reduction in photorelaxation by the guanylyl cyclase inhibitor and NO scavenger agents indicates involvement of both NO and cGMP in photorelaxation. In addition the sensitivity of photorelaxation to the voltage-gated potassium channel (K(v)) inhibitor, 4-aminopyridine, indicates that photorelaxation is mediated via a NO/cGMP-dependent, and, perhaps, direct light, activation of K(v) channels.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalEuropean Journal of Pharmacology
Volume347
Issue number2-3
DOIs
Publication statusPublished - 24 Apr 1998

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Keywords

  • Guanylyl cyclase
  • K channel
  • Nitric oxide (NO)
  • Nitrosothiol
  • Photorelaxation

ASJC Scopus subject areas

  • Pharmacology

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