Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques.

S. M. Wilson, J. D. Pediani, W. H. Ko, Douglas Bovell, S. Kitson, I. Montgomery, U. M. Brown, G. L. Smith, H. Y. Elder, D. M. Jenkinson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

When sweat glands isolated from samples of horse skin were explanted and cultured under favourable conditions, they could exhibit cellular outgrowth. This growth could be maintained for 2-4 weeks and these primary cultures were then disaggregated and the resultant cell suspensions used to initiate epithelial cell lines. Secretion from intact equine sweat glands is regulated by beta 2-adrenoceptors and appears to be mediated by cyclic AMP, but there is evidence that calcium may also play a role. Adrenaline could increase the cyclic AMP content of the cultured cells and this response was mediated by beta 2-adrenoceptors. Adrenaline was also able to evoke a small increase in intracellular free calcium ([Ca2+]i) but the pharmacology of this response remains obscure. Adrenaline thus activates at least two potentially important second-messenger signalling pathways which have the capacity to interact, because adrenaline-evoked cyclic AMP formation was inhibited if [Ca2+]i was raised with ionomycin. The chloride permeability of mammalian epithelial cells characteristically rises during secretion, and adrenaline could increase chloride permeability in the cultured epithelia but the cells did not contain cyclic-AMP-dependent chloride channels and so this response was mediated by [Ca2+]i.

Original languageEnglish
Pages (from-to)279-299
Number of pages21
JournalJournal of Experimental Biology
Volume183
Publication statusPublished - Oct 1993
Externally publishedYes

Fingerprint

sweat glands
Sweat Glands
epinephrine
secretion
Epinephrine
Horses
cyclic AMP
cell culture
epithelium
Epithelium
Cell Culture Techniques
Cyclic AMP
chloride
calcium
horses
permeability
Adrenergic Receptors
Chlorides
horse
Cultured Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Wilson, S. M., Pediani, J. D., Ko, W. H., Bovell, D., Kitson, S., Montgomery, I., ... Jenkinson, D. M. (1993). Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques. Journal of Experimental Biology, 183, 279-299.

Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques. / Wilson, S. M.; Pediani, J. D.; Ko, W. H.; Bovell, Douglas; Kitson, S.; Montgomery, I.; Brown, U. M.; Smith, G. L.; Elder, H. Y.; Jenkinson, D. M.

In: Journal of Experimental Biology, Vol. 183, 10.1993, p. 279-299.

Research output: Contribution to journalArticle

Wilson, SM, Pediani, JD, Ko, WH, Bovell, D, Kitson, S, Montgomery, I, Brown, UM, Smith, GL, Elder, HY & Jenkinson, DM 1993, 'Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques.', Journal of Experimental Biology, vol. 183, pp. 279-299.
Wilson, S. M. ; Pediani, J. D. ; Ko, W. H. ; Bovell, Douglas ; Kitson, S. ; Montgomery, I. ; Brown, U. M. ; Smith, G. L. ; Elder, H. Y. ; Jenkinson, D. M. / Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques. In: Journal of Experimental Biology. 1993 ; Vol. 183. pp. 279-299.
@article{50bd35c2b56e41b4b570990333c0e349,
title = "Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques.",
abstract = "When sweat glands isolated from samples of horse skin were explanted and cultured under favourable conditions, they could exhibit cellular outgrowth. This growth could be maintained for 2-4 weeks and these primary cultures were then disaggregated and the resultant cell suspensions used to initiate epithelial cell lines. Secretion from intact equine sweat glands is regulated by beta 2-adrenoceptors and appears to be mediated by cyclic AMP, but there is evidence that calcium may also play a role. Adrenaline could increase the cyclic AMP content of the cultured cells and this response was mediated by beta 2-adrenoceptors. Adrenaline was also able to evoke a small increase in intracellular free calcium ([Ca2+]i) but the pharmacology of this response remains obscure. Adrenaline thus activates at least two potentially important second-messenger signalling pathways which have the capacity to interact, because adrenaline-evoked cyclic AMP formation was inhibited if [Ca2+]i was raised with ionomycin. The chloride permeability of mammalian epithelial cells characteristically rises during secretion, and adrenaline could increase chloride permeability in the cultured epithelia but the cells did not contain cyclic-AMP-dependent chloride channels and so this response was mediated by [Ca2+]i.",
author = "Wilson, {S. M.} and Pediani, {J. D.} and Ko, {W. H.} and Douglas Bovell and S. Kitson and I. Montgomery and Brown, {U. M.} and Smith, {G. L.} and Elder, {H. Y.} and Jenkinson, {D. M.}",
year = "1993",
month = "10",
language = "English",
volume = "183",
pages = "279--299",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",

}

TY - JOUR

T1 - Investigation of stimulus-secretion coupling in equine sweat gland epithelia using cell culture techniques.

AU - Wilson, S. M.

AU - Pediani, J. D.

AU - Ko, W. H.

AU - Bovell, Douglas

AU - Kitson, S.

AU - Montgomery, I.

AU - Brown, U. M.

AU - Smith, G. L.

AU - Elder, H. Y.

AU - Jenkinson, D. M.

PY - 1993/10

Y1 - 1993/10

N2 - When sweat glands isolated from samples of horse skin were explanted and cultured under favourable conditions, they could exhibit cellular outgrowth. This growth could be maintained for 2-4 weeks and these primary cultures were then disaggregated and the resultant cell suspensions used to initiate epithelial cell lines. Secretion from intact equine sweat glands is regulated by beta 2-adrenoceptors and appears to be mediated by cyclic AMP, but there is evidence that calcium may also play a role. Adrenaline could increase the cyclic AMP content of the cultured cells and this response was mediated by beta 2-adrenoceptors. Adrenaline was also able to evoke a small increase in intracellular free calcium ([Ca2+]i) but the pharmacology of this response remains obscure. Adrenaline thus activates at least two potentially important second-messenger signalling pathways which have the capacity to interact, because adrenaline-evoked cyclic AMP formation was inhibited if [Ca2+]i was raised with ionomycin. The chloride permeability of mammalian epithelial cells characteristically rises during secretion, and adrenaline could increase chloride permeability in the cultured epithelia but the cells did not contain cyclic-AMP-dependent chloride channels and so this response was mediated by [Ca2+]i.

AB - When sweat glands isolated from samples of horse skin were explanted and cultured under favourable conditions, they could exhibit cellular outgrowth. This growth could be maintained for 2-4 weeks and these primary cultures were then disaggregated and the resultant cell suspensions used to initiate epithelial cell lines. Secretion from intact equine sweat glands is regulated by beta 2-adrenoceptors and appears to be mediated by cyclic AMP, but there is evidence that calcium may also play a role. Adrenaline could increase the cyclic AMP content of the cultured cells and this response was mediated by beta 2-adrenoceptors. Adrenaline was also able to evoke a small increase in intracellular free calcium ([Ca2+]i) but the pharmacology of this response remains obscure. Adrenaline thus activates at least two potentially important second-messenger signalling pathways which have the capacity to interact, because adrenaline-evoked cyclic AMP formation was inhibited if [Ca2+]i was raised with ionomycin. The chloride permeability of mammalian epithelial cells characteristically rises during secretion, and adrenaline could increase chloride permeability in the cultured epithelia but the cells did not contain cyclic-AMP-dependent chloride channels and so this response was mediated by [Ca2+]i.

UR - http://www.scopus.com/inward/record.url?scp=0027674151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027674151&partnerID=8YFLogxK

M3 - Article

VL - 183

SP - 279

EP - 299

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

ER -