Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan

Hina Sarwath, Devendra Bansal, Nazik Elmalaika Husain, Mahmoud Mohamed, Ali Sultan, Shahinaz Bedri

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Cervical cancer is the fourth most common cancer in women worldwide with highest incidence reported in Eastern Africa in 2012. The primary goal of this study was to study the expression of p16INK4a in squamous cell carcinoma (SCC) of the cervix by immunohistochemistry (IHC) and determine relation with clinico-pathological parameters. This study further explored the correlation of p16INK4a immunostaining with another proliferation marker, Ki-67 and to study if human papillomavirus (HPV) IHC can be used as a marker for detection of virus in high-grade dysplasia. Methods: A total of 90 samples, diagnosed for cervical cancer, were included in the study. Fixed Paraffin Embedded (FFPE) tissue sections were stained with anti-p16INK4a, anti-Ki-67 and anti-HPV antibodies using automated immunohistochemistry platform (ASLink 48-DAKO). Results: Immunohistochemical protein expression of p16INK4a positivity was found to be highest in SCC (92.2%, n = 71) than other HPV tumors (76.9%, n = 10). The majority of cases (97.4%) were p16INK4a positive in the age group 41-60 years. In addition, a statistically significant difference between p16INK4a and HPV was observed among total cervical tumor cases and SCC cases. Conclusions: As expected staining of invasive cervical cancer with anti-HPV showed rare positivity because HPV heralds active infection in dysplastic lesions and not of frank cervical carcinoma. In contrast, anti-p16INK4a IHC results showed positive correlation in SCC and other cervical tumors.

Original languageEnglish
Article number50
JournalInfectious Agents and Cancer
Volume12
Issue number1
DOIs
Publication statusPublished - 30 Sep 2017

Fingerprint

Sudan
Uterine Cervical Neoplasms
Biomarkers
Squamous Cell Carcinoma
Immunohistochemistry
Neoplasms
Cyclin-Dependent Kinase Inhibitor p16
Eastern Africa
Cervix Uteri
Paraffin
Age Groups
Staining and Labeling
Viruses
Carcinoma
Antibodies
Incidence
Infection

Keywords

  • Cervical cancer
  • Human Papillomavirus
  • Immunohistochemistry
  • Ki-67
  • p16
  • Sudan

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Infectious Diseases
  • Cancer Research

Cite this

Introduction of p16INK4a as a surrogate biomarker for HPV in women with invasive cervical cancer in Sudan. / Sarwath, Hina; Bansal, Devendra; Husain, Nazik Elmalaika; Mohamed, Mahmoud; Sultan, Ali; Bedri, Shahinaz.

In: Infectious Agents and Cancer, Vol. 12, No. 1, 50, 30.09.2017.

Research output: Contribution to journalArticle

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abstract = "Background: Cervical cancer is the fourth most common cancer in women worldwide with highest incidence reported in Eastern Africa in 2012. The primary goal of this study was to study the expression of p16INK4a in squamous cell carcinoma (SCC) of the cervix by immunohistochemistry (IHC) and determine relation with clinico-pathological parameters. This study further explored the correlation of p16INK4a immunostaining with another proliferation marker, Ki-67 and to study if human papillomavirus (HPV) IHC can be used as a marker for detection of virus in high-grade dysplasia. Methods: A total of 90 samples, diagnosed for cervical cancer, were included in the study. Fixed Paraffin Embedded (FFPE) tissue sections were stained with anti-p16INK4a, anti-Ki-67 and anti-HPV antibodies using automated immunohistochemistry platform (ASLink 48-DAKO). Results: Immunohistochemical protein expression of p16INK4a positivity was found to be highest in SCC (92.2{\%}, n = 71) than other HPV tumors (76.9{\%}, n = 10). The majority of cases (97.4{\%}) were p16INK4a positive in the age group 41-60 years. In addition, a statistically significant difference between p16INK4a and HPV was observed among total cervical tumor cases and SCC cases. Conclusions: As expected staining of invasive cervical cancer with anti-HPV showed rare positivity because HPV heralds active infection in dysplastic lesions and not of frank cervical carcinoma. In contrast, anti-p16INK4a IHC results showed positive correlation in SCC and other cervical tumors.",
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