Intragraft TGF-β1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy

Vijay K. Sharma, Roxana M. Bologa, Guo Ping Xu, Baogui Li, Janet Mouradian, John Wang, David Serur, Venkateswara Rao, Manikkam Suthanthiran

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Chronic allograft nephropathy is a relentlessly progressive process and a major cause of long-term graft dysfunction and ultimate failure. Interstitial fibrosis, tubular atrophy, and glomerular and vascular lesions characterize this mechanistically unresolved disorder. Given the prominent role of TGF-β1 in tissue repair and in fibrosis, we have explored the hypothesis that fibrosis and chronic allograft nephropathy would be distinguished by intragraft TGF-β1 mRNA expression. This postulate was tested by mRNA phenotyping of RNA isolated from 127 human renal allograft biopsies. Reverse transcription assisted polymerase chain reaction was used to amplify and identify ingraft gene expression. Our investigation demonstrated a significant correlation between intragraft TGF-β1 mRNA display and renal allograft interstitial fibrosis and chronic allograft nephropathy. In contrast, intragraft expression of mRNA encoding immunoregulatory cytokines, IL-2, IFN-γ, IL-4, IL-10, or cytotoxic attack molecules, granzyme B and perforin was not a correlate of interstitial fibrosis or chronic allograft nephropathy. Our studies identify, for the first time, a significant association between intragraft TGF-β1 mRNA expression and renal allograft interstitial fibrosis, and advance a candidate molecular mechanism for chronic allograft nephropathy.

Original languageEnglish
Pages (from-to)1297-1303
Number of pages7
JournalKidney International
Volume49
Issue number5
Publication statusPublished - 1 Jan 1996
Externally publishedYes

Fingerprint

Allografts
Fibrosis
Messenger RNA
Kidney
Interleukin-4
Interleukin-10
Reverse Transcription
Atrophy
Interleukin-2
Blood Vessels
RNA
Cytokines
Transplants
Biopsy
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Nephrology

Cite this

Sharma, V. K., Bologa, R. M., Xu, G. P., Li, B., Mouradian, J., Wang, J., ... Suthanthiran, M. (1996). Intragraft TGF-β1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy. Kidney International, 49(5), 1297-1303.

Intragraft TGF-β1 mRNA : A correlate of interstitial fibrosis and chronic allograft nephropathy. / Sharma, Vijay K.; Bologa, Roxana M.; Xu, Guo Ping; Li, Baogui; Mouradian, Janet; Wang, John; Serur, David; Rao, Venkateswara; Suthanthiran, Manikkam.

In: Kidney International, Vol. 49, No. 5, 01.01.1996, p. 1297-1303.

Research output: Contribution to journalArticle

Sharma, VK, Bologa, RM, Xu, GP, Li, B, Mouradian, J, Wang, J, Serur, D, Rao, V & Suthanthiran, M 1996, 'Intragraft TGF-β1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy', Kidney International, vol. 49, no. 5, pp. 1297-1303.
Sharma VK, Bologa RM, Xu GP, Li B, Mouradian J, Wang J et al. Intragraft TGF-β1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy. Kidney International. 1996 Jan 1;49(5):1297-1303.
Sharma, Vijay K. ; Bologa, Roxana M. ; Xu, Guo Ping ; Li, Baogui ; Mouradian, Janet ; Wang, John ; Serur, David ; Rao, Venkateswara ; Suthanthiran, Manikkam. / Intragraft TGF-β1 mRNA : A correlate of interstitial fibrosis and chronic allograft nephropathy. In: Kidney International. 1996 ; Vol. 49, No. 5. pp. 1297-1303.
@article{4f595b4599e24683a5863738bf6187c9,
title = "Intragraft TGF-β1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy",
abstract = "Chronic allograft nephropathy is a relentlessly progressive process and a major cause of long-term graft dysfunction and ultimate failure. Interstitial fibrosis, tubular atrophy, and glomerular and vascular lesions characterize this mechanistically unresolved disorder. Given the prominent role of TGF-β1 in tissue repair and in fibrosis, we have explored the hypothesis that fibrosis and chronic allograft nephropathy would be distinguished by intragraft TGF-β1 mRNA expression. This postulate was tested by mRNA phenotyping of RNA isolated from 127 human renal allograft biopsies. Reverse transcription assisted polymerase chain reaction was used to amplify and identify ingraft gene expression. Our investigation demonstrated a significant correlation between intragraft TGF-β1 mRNA display and renal allograft interstitial fibrosis and chronic allograft nephropathy. In contrast, intragraft expression of mRNA encoding immunoregulatory cytokines, IL-2, IFN-γ, IL-4, IL-10, or cytotoxic attack molecules, granzyme B and perforin was not a correlate of interstitial fibrosis or chronic allograft nephropathy. Our studies identify, for the first time, a significant association between intragraft TGF-β1 mRNA expression and renal allograft interstitial fibrosis, and advance a candidate molecular mechanism for chronic allograft nephropathy.",
author = "Sharma, {Vijay K.} and Bologa, {Roxana M.} and Xu, {Guo Ping} and Baogui Li and Janet Mouradian and John Wang and David Serur and Venkateswara Rao and Manikkam Suthanthiran",
year = "1996",
month = "1",
day = "1",
language = "English",
volume = "49",
pages = "1297--1303",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Intragraft TGF-β1 mRNA

T2 - A correlate of interstitial fibrosis and chronic allograft nephropathy

AU - Sharma, Vijay K.

AU - Bologa, Roxana M.

AU - Xu, Guo Ping

AU - Li, Baogui

AU - Mouradian, Janet

AU - Wang, John

AU - Serur, David

AU - Rao, Venkateswara

AU - Suthanthiran, Manikkam

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Chronic allograft nephropathy is a relentlessly progressive process and a major cause of long-term graft dysfunction and ultimate failure. Interstitial fibrosis, tubular atrophy, and glomerular and vascular lesions characterize this mechanistically unresolved disorder. Given the prominent role of TGF-β1 in tissue repair and in fibrosis, we have explored the hypothesis that fibrosis and chronic allograft nephropathy would be distinguished by intragraft TGF-β1 mRNA expression. This postulate was tested by mRNA phenotyping of RNA isolated from 127 human renal allograft biopsies. Reverse transcription assisted polymerase chain reaction was used to amplify and identify ingraft gene expression. Our investigation demonstrated a significant correlation between intragraft TGF-β1 mRNA display and renal allograft interstitial fibrosis and chronic allograft nephropathy. In contrast, intragraft expression of mRNA encoding immunoregulatory cytokines, IL-2, IFN-γ, IL-4, IL-10, or cytotoxic attack molecules, granzyme B and perforin was not a correlate of interstitial fibrosis or chronic allograft nephropathy. Our studies identify, for the first time, a significant association between intragraft TGF-β1 mRNA expression and renal allograft interstitial fibrosis, and advance a candidate molecular mechanism for chronic allograft nephropathy.

AB - Chronic allograft nephropathy is a relentlessly progressive process and a major cause of long-term graft dysfunction and ultimate failure. Interstitial fibrosis, tubular atrophy, and glomerular and vascular lesions characterize this mechanistically unresolved disorder. Given the prominent role of TGF-β1 in tissue repair and in fibrosis, we have explored the hypothesis that fibrosis and chronic allograft nephropathy would be distinguished by intragraft TGF-β1 mRNA expression. This postulate was tested by mRNA phenotyping of RNA isolated from 127 human renal allograft biopsies. Reverse transcription assisted polymerase chain reaction was used to amplify and identify ingraft gene expression. Our investigation demonstrated a significant correlation between intragraft TGF-β1 mRNA display and renal allograft interstitial fibrosis and chronic allograft nephropathy. In contrast, intragraft expression of mRNA encoding immunoregulatory cytokines, IL-2, IFN-γ, IL-4, IL-10, or cytotoxic attack molecules, granzyme B and perforin was not a correlate of interstitial fibrosis or chronic allograft nephropathy. Our studies identify, for the first time, a significant association between intragraft TGF-β1 mRNA expression and renal allograft interstitial fibrosis, and advance a candidate molecular mechanism for chronic allograft nephropathy.

UR - http://www.scopus.com/inward/record.url?scp=0029937385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029937385&partnerID=8YFLogxK

M3 - Article

C2 - 8731094

AN - SCOPUS:0029937385

VL - 49

SP - 1297

EP - 1303

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 5

ER -