Intragraft expression of IL-10 messenger RNA: A novel correlate of renal allograft rejection

Guo Ping Xu, Vijay K. Sharma, Baogui Li, Roxana Bologa, Yi Li, Janet Mouradian, John Wang, David Serur, Venkateswara Rao, Kurt H. Stenzel, Manikkam Suthanthiran

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A major conceptual advance is the formulation that type I cytokines (such as IL-2 and IFN-γ) enhance cellular immunity and are host-protective, and that type II cytokines (such as IL-4 and IL-10) dampen cellular immunity and facilitate the progression of infection. We have explored the intragraft expression of type I and type II cytokines during human renal allograft rejection. RNA was isolated from 98 allograft biopsies, and reverse transcription-PCR was used to amplify and identify intragraft expression of mRNA encoding IL-2, IFN-γ, IL-4, or IL-10. Intragraft expression of IL-7 mRNA and TGF-β1 mRNA was also investigated. Our investigation demonstrated that: (a) intragraft expression of IL-10 mRNA and IL-2 mRNA are significant correlates of acute rejection; (b) IL-4, IL-7, IFN-γ and TGF-β1 mRNA expression do not correlate with acute rejection; and (c) IL-10 does not prevent in vivo expression of IFN-γ, IL-2, IL-7, or TGF-β1. Our studies identify, for the first time, a significant association between intragraft IL-10 mRNA expression and acute rejection, and suggest that treatment strategies capable of constraining IL-10 expression might be of value in the prevention of acute rejection.

Original languageEnglish
Pages (from-to)1504-1507
Number of pages4
JournalKidney International
Issue number5
Publication statusPublished - 1 Jan 1995
Externally publishedYes


ASJC Scopus subject areas

  • Nephrology

Cite this

Xu, G. P., Sharma, V. K., Li, B., Bologa, R., Li, Y., Mouradian, J., Wang, J., Serur, D., Rao, V., Stenzel, K. H., & Suthanthiran, M. (1995). Intragraft expression of IL-10 messenger RNA: A novel correlate of renal allograft rejection. Kidney International, 48(5), 1504-1507.