Intracellular trafficking of adenovirus

Many means to many ends

Philip L. Leopold, Ronald Crystal

Research output: Contribution to journalReview article

72 Citations (Scopus)

Abstract

The intracellular trafficking of adenovirus capsids has been described mainly through observations of trafficking by capsids from subgroup C adenoviruses in transformed cell lines. The basic elements of the trafficking pathway include high affinity binding of the adenovirus capsid to receptors at the cell surface, internalization by endocytosis, lysis of the endosomal membrane resulting in escape to the cytosol, trafficking along microtubules, binding to the nuclear envelope, and insertion of the viral genome through the nuclear pore. The net effect of this basic pathway is to deliver the adenovirus genome to the nucleus in a highly efficient manner with greater than 80% of the genome reaching the nucleus in approximately 1 h. However, exceptions to this trafficking pattern have been noted, including: (1) variations based on adenovirus serotype; (2) variations based on target cell type; and (3) variations based on cell physiology. This review summarizes the classical adenovirus infection pathway along with the exceptions to that trafficking pathway, providing an overview of intracellular trafficking of adenovirus.

Original languageEnglish
Pages (from-to)810-821
Number of pages12
JournalAdvanced Drug Delivery Reviews
Volume59
Issue number8
DOIs
Publication statusPublished - 10 Aug 2007
Externally publishedYes

Fingerprint

Adenoviridae
Capsid
Genome
Adenoviridae Infections
Nuclear Pore
Cell Physiological Phenomena
Transformed Cell Line
Viral Genome
Nuclear Envelope
Cell Surface Receptors
Endocytosis
Microtubules
Cytosol
Membranes

Keywords

  • Adenoviruses
  • Cell physiology
  • Endocytosis
  • Intracellular trafficking
  • Serum proteins
  • Viral infection
  • Viral receptors

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Intracellular trafficking of adenovirus : Many means to many ends. / Leopold, Philip L.; Crystal, Ronald.

In: Advanced Drug Delivery Reviews, Vol. 59, No. 8, 10.08.2007, p. 810-821.

Research output: Contribution to journalReview article

@article{2822cc1de3054e6c9850bdca5213cac2,
title = "Intracellular trafficking of adenovirus: Many means to many ends",
abstract = "The intracellular trafficking of adenovirus capsids has been described mainly through observations of trafficking by capsids from subgroup C adenoviruses in transformed cell lines. The basic elements of the trafficking pathway include high affinity binding of the adenovirus capsid to receptors at the cell surface, internalization by endocytosis, lysis of the endosomal membrane resulting in escape to the cytosol, trafficking along microtubules, binding to the nuclear envelope, and insertion of the viral genome through the nuclear pore. The net effect of this basic pathway is to deliver the adenovirus genome to the nucleus in a highly efficient manner with greater than 80{\%} of the genome reaching the nucleus in approximately 1 h. However, exceptions to this trafficking pattern have been noted, including: (1) variations based on adenovirus serotype; (2) variations based on target cell type; and (3) variations based on cell physiology. This review summarizes the classical adenovirus infection pathway along with the exceptions to that trafficking pathway, providing an overview of intracellular trafficking of adenovirus.",
keywords = "Adenoviruses, Cell physiology, Endocytosis, Intracellular trafficking, Serum proteins, Viral infection, Viral receptors",
author = "Leopold, {Philip L.} and Ronald Crystal",
year = "2007",
month = "8",
day = "10",
doi = "10.1016/j.addr.2007.06.007",
language = "English",
volume = "59",
pages = "810--821",
journal = "Advanced Drug Delivery Reviews",
issn = "0169-409X",
publisher = "Elsevier",
number = "8",

}

TY - JOUR

T1 - Intracellular trafficking of adenovirus

T2 - Many means to many ends

AU - Leopold, Philip L.

AU - Crystal, Ronald

PY - 2007/8/10

Y1 - 2007/8/10

N2 - The intracellular trafficking of adenovirus capsids has been described mainly through observations of trafficking by capsids from subgroup C adenoviruses in transformed cell lines. The basic elements of the trafficking pathway include high affinity binding of the adenovirus capsid to receptors at the cell surface, internalization by endocytosis, lysis of the endosomal membrane resulting in escape to the cytosol, trafficking along microtubules, binding to the nuclear envelope, and insertion of the viral genome through the nuclear pore. The net effect of this basic pathway is to deliver the adenovirus genome to the nucleus in a highly efficient manner with greater than 80% of the genome reaching the nucleus in approximately 1 h. However, exceptions to this trafficking pattern have been noted, including: (1) variations based on adenovirus serotype; (2) variations based on target cell type; and (3) variations based on cell physiology. This review summarizes the classical adenovirus infection pathway along with the exceptions to that trafficking pathway, providing an overview of intracellular trafficking of adenovirus.

AB - The intracellular trafficking of adenovirus capsids has been described mainly through observations of trafficking by capsids from subgroup C adenoviruses in transformed cell lines. The basic elements of the trafficking pathway include high affinity binding of the adenovirus capsid to receptors at the cell surface, internalization by endocytosis, lysis of the endosomal membrane resulting in escape to the cytosol, trafficking along microtubules, binding to the nuclear envelope, and insertion of the viral genome through the nuclear pore. The net effect of this basic pathway is to deliver the adenovirus genome to the nucleus in a highly efficient manner with greater than 80% of the genome reaching the nucleus in approximately 1 h. However, exceptions to this trafficking pattern have been noted, including: (1) variations based on adenovirus serotype; (2) variations based on target cell type; and (3) variations based on cell physiology. This review summarizes the classical adenovirus infection pathway along with the exceptions to that trafficking pathway, providing an overview of intracellular trafficking of adenovirus.

KW - Adenoviruses

KW - Cell physiology

KW - Endocytosis

KW - Intracellular trafficking

KW - Serum proteins

KW - Viral infection

KW - Viral receptors

UR - http://www.scopus.com/inward/record.url?scp=34548236927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548236927&partnerID=8YFLogxK

U2 - 10.1016/j.addr.2007.06.007

DO - 10.1016/j.addr.2007.06.007

M3 - Review article

VL - 59

SP - 810

EP - 821

JO - Advanced Drug Delivery Reviews

JF - Advanced Drug Delivery Reviews

SN - 0169-409X

IS - 8

ER -