Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype

Yueh Chun Li, Charles Lee, Despina Sanoudou, Tzong Hsiung Hsu, Shuan Yow Li, Chyi Chyang Lin

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    Abstract

    A number of repetitive DNA clones were generated from PCR amplifications of Indian muntjac genomic DNA using primer sequences derived from a white tailed deer satellite II DNA sequence. One clone (Mmv-0.7) was characterized and shown to be a cervid satellite II DNA clone. Multiple colored FISH studies with cervid satellite I (C5) and this satellite II clone (Mmv-0.7) to Chinese muntjac metaphase chromosomes localized both satellite DNAs at the pericentromeric regions of all chromosomes except for chromosome 3 and the Y chromosome, whereas chromosome 3 exhibited pericentromeric satellite II DNA only. Where distinguishable, the pericentromeric satellite II signals appeared terminally oriented with respect to satellite I. Six pairs of Chinese muntjac autosomes had interstitial satellite I sites with four of these autosomal pairs (chromosomes 1, 2 and two other smaller autosomal pairs) also exhibiting interstitial satellite II signals. An interstitial site on the X chromosome was found to have satellite II signals. For the Indian muntjac chromosomes, FISH studies revealed a pericentromeric hybridization for satellites I and II as well as 27 distinct interstitial hybridization sites, each having at least one of the satellite DNAs. These data were used to more precisely define the chromosome fusion-associated breakpoints that presumably led to the formation of the present-day Indian muntjac karyotype. It further hints at the possibility that the Indian muntjac karyotype may have evolved directly from a 2n = 70 ancestral karyotype rather than from an intermediate 2n = 46 Chinese muntjac-like karyotype.

    Original languageEnglish
    Pages (from-to)363-373
    Number of pages11
    JournalChromosome Research
    Volume8
    Issue number5
    DOIs
    Publication statusPublished - 20 Sep 2000

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    Keywords

    • Centromeric DNA
    • Cervid satellite DNA
    • Karyotypic evolution
    • Muntjac chromosomes

    ASJC Scopus subject areas

    • Genetics

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