Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis

Carles Pucharcos, Xavier Estivill, Susana De La Luna

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Intersectin 1 (ITSN1) is a binding partner of dynamin that has been shown to participate in clathrin-mediated endocytosis. Here we report the characterization of a new human gene, ITSN2, highly similar to ITSN1. Alternative splicing of ITSN2 generates a short isoform with two EH domains, a coiled-coil region and five SH3 domains, and a longer isoform containing extra carboxy domains (DH, PH and C2 domains), suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases. ITSN2 expression analysis indicates that it is widely expressed in human tissues. Intersectin 2 isoforms show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin internalization.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalFEBS Letters
Issue number1-2
Publication statusPublished - 28 Jul 2000



  • Alternative splicing
  • Clathrin
  • Endocytosis
  • Intersectin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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