Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis

Carles Pucharcos, Xavier P. Estivill, Susana De La Luna

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Intersectin 1 (ITSN1) is a binding partner of dynamin that has been shown to participate in clathrin-mediated endocytosis. Here we report the characterization of a new human gene, ITSN2, highly similar to ITSN1. Alternative splicing of ITSN2 generates a short isoform with two EH domains, a coiled-coil region and five SH3 domains, and a longer isoform containing extra carboxy domains (DH, PH and C2 domains), suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases. ITSN2 expression analysis indicates that it is widely expressed in human tissues. Intersectin 2 isoforms show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin internalization.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalFEBS Letters
Volume478
Issue number1-2
DOIs
Publication statusPublished - 28 Jul 2000
Externally publishedYes

Fingerprint

Clathrin
Endocytosis
Protein Isoforms
Proteins
Rho Guanine Nucleotide Exchange Factors
Dynamins
Guanine Nucleotide Exchange Factors
rho GTP-Binding Proteins
src Homology Domains
GTP Phosphohydrolases
Alternative Splicing
Transferrin
Machinery
Genes
Tissue
intersectin 1

Keywords

  • Alternative splicing
  • Clathrin
  • Endocytosis
  • Intersectin

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis. / Pucharcos, Carles; Estivill, Xavier P.; De La Luna, Susana.

In: FEBS Letters, Vol. 478, No. 1-2, 28.07.2000, p. 43-51.

Research output: Contribution to journalArticle

Pucharcos, Carles ; Estivill, Xavier P. ; De La Luna, Susana. / Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis. In: FEBS Letters. 2000 ; Vol. 478, No. 1-2. pp. 43-51.
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