Interrogation of living myocardium in multiple static deformation states with diffusion tensor and diffusion spectrum imaging

Maelene Lohezic, Irvin Teh, Christian Bollensdorff, Rémi Peyronnet, Patrick W. Hales, Vicente Grau, Peter Kohl, Jürgen E. Schneider

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Diffusion tensor magnetic resonance imaging (MRI) reveals valuable insights into tissue histo-anatomy and microstructure, and has steadily gained traction in the cardiac community. Its wider use in small animal cardiac imaging invivo has been constrained by its extreme sensitivity to motion, exaggerated by the high heart rates usually seen in rodents. Imaging of the isolated heart eliminates respiratory motion and, if conducted on arrested hearts, cardiac pulsation. This serves as an important intermediate step for basic and translational studies. However, investigating the micro-structural basis of cardiac deformation in the same heart requires observations in different deformation states. Here, we illustrate the imaging of isolated rat hearts in three mechanical states mimicking diastole (cardioplegic arrest), left-ventricular (LV) volume overload (cardioplegic arrest plus LV balloon inflation), and peak systole (lithium-induced contracture). An optimised MRI-compatible Langendorff perfusion setup with the radio-frequency (RF) coil integrated into the wet chamber was developed for use in a 9.4T horizontal bore scanner. Signal-to-noise ratio improved significantly, by 75% compared to a previous design with external RF coil, and stability tests showed no significant changes in mean T1, T2 or LV wall thickness over a 170min period. In contracture, we observed a significant reduction in mean fractional anisotropy from 0.32±0.02 to 0.28±0.02, as well as a significant rightward shift in helix angles with a decrease in the proportion of left-handed fibres, as referring to the locally prevailing cell orientation in the heart, from 24.9% to 23.3%, and an increase in the proportion of right-handed fibres from 25.5% to 28.4%. LV overload, in contrast, gave rise to a decrease in the proportion of left-handed fibres from 24.9% to 21.4% and an increase in the proportion of right-handed fibres from 25.5% to 26.0%. The modified perfusion and coil setup offers better performance and control over cardiac contraction states. We subsequently performed high-resolution diffusion spectrum imaging (DSI) and 3D whole heart fibre tracking in fixed exvivo rat hearts in slack state and contracture. As a model-free method, DSI augmented the measurements of water diffusion by also informing on multiple intra-voxel diffusion orientations and non-Gaussian diffusion. This enabled us to identify the transition from right- to left-handed fibres from the subendocardium to the subepicardium, as well as voxels in apical regions that were traversed by multiple fibres. We observed that both the mean generalised fractional anisotropy and mean kurtosis were lower in hearts in contracture compared to the slack state, by 23% and 9.3%, respectively. While its heavy acquisition burden currently limits the application of DSI invivo, ongoing work in acceleration techniques may enable its use in live animals and patients. This would provide access to the as yet unexplored dimension of non-Gaussian diffusion that could serve as a highly sensitive marker of cardiac micro-structural integrity.

    Original languageEnglish
    Pages (from-to)213-225
    Number of pages13
    JournalProgress in Biophysics and Molecular Biology
    Volume115
    Issue number2-3
    DOIs
    Publication statusPublished - 1 Jan 2014

    Fingerprint

    Diffusion Tensor Imaging
    Myocardium
    Contracture
    Anisotropy
    Radio
    Perfusion
    Diffusion Magnetic Resonance Imaging
    Diastole
    Systole
    Economic Inflation
    Traction
    Signal-To-Noise Ratio
    Lithium
    Rodentia
    Anatomy
    Heart Rate
    Magnetic Resonance Imaging
    Water

    Keywords

    • Cardiac MRI
    • Diffusion spectrum imaging
    • Diffusion tensor imaging
    • Isolated heart
    • Langendorff perfusion
    • Magnetic resonance imaging

    ASJC Scopus subject areas

    • Molecular Biology
    • Biophysics

    Cite this

    Interrogation of living myocardium in multiple static deformation states with diffusion tensor and diffusion spectrum imaging. / Lohezic, Maelene; Teh, Irvin; Bollensdorff, Christian; Peyronnet, Rémi; Hales, Patrick W.; Grau, Vicente; Kohl, Peter; Schneider, Jürgen E.

    In: Progress in Biophysics and Molecular Biology, Vol. 115, No. 2-3, 01.01.2014, p. 213-225.

    Research output: Contribution to journalArticle

    Lohezic, Maelene ; Teh, Irvin ; Bollensdorff, Christian ; Peyronnet, Rémi ; Hales, Patrick W. ; Grau, Vicente ; Kohl, Peter ; Schneider, Jürgen E. / Interrogation of living myocardium in multiple static deformation states with diffusion tensor and diffusion spectrum imaging. In: Progress in Biophysics and Molecular Biology. 2014 ; Vol. 115, No. 2-3. pp. 213-225.
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    AU - Teh, Irvin

    AU - Bollensdorff, Christian

    AU - Peyronnet, Rémi

    AU - Hales, Patrick W.

    AU - Grau, Vicente

    AU - Kohl, Peter

    AU - Schneider, Jürgen E.

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