Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation

Wassim El-Jouni, Shirley Haun, Khaled Machaca

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A transient increase in intracellular Ca2+ is the universal signal for egg activation at fertilization. Eggs acquire the ability to mount the specialized fertilization-specific Ca2+ signal during oocyte maturation. The first Ca2+ transient following sperm entry in vertebrate eggs has a slow rising phase followed by a sustained plateau. The molecular determinants of the sustained plateau are poorly understood. We have recently shown that a critical determinant of Ca2+ signaling differentiation during oocyte maturation is internalization of the plasma membrane calcium ATPase (PMCA). PMCA internalization is representative of endocytosis of several integral membrane proteins during oocyte maturation, a requisite process for early embryogenesis. Here we investigate the mechanisms regulating PMCA internalization. To track PMCA trafficking in live cells we cloned a full-length cDNA of Xenopus PMCA1, and show that GFP-tagged PMCA traffics in a similar fashion to endogenous PMCA. Functional data show that MPF activation during oocyte maturation is required for full PMCA internalization. Pharmacological and co-localization studies argue that PMCA is internalized through a lipid raft endocytic pathway. Deletion analysis reveal a requirement for the N-terminal cytoplasmic domain for efficient internalization. Together these studies define the mechanistic requirements for PMCA internalization during oocyte maturation.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalDevelopmental Biology
Volume324
Issue number1
DOIs
Publication statusPublished - 1 Dec 2008

Fingerprint

Plasma Membrane Calcium-Transporting ATPases
Calcium-Transporting ATPases
Xenopus
Oocytes
Cell Membrane
Fertilization
Eggs
Endocytosis
Embryonic Development
Ovum
Vertebrates
Spermatozoa
Membrane Proteins
Complementary DNA

Keywords

  • Calcium
  • Endocytosis
  • Lipid-rafts
  • Oocyte maturation
  • Plasma-membrane Ca ATPase (PMCA)
  • Trafficking
  • Xenopus laevis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation. / El-Jouni, Wassim; Haun, Shirley; Machaca, Khaled.

In: Developmental Biology, Vol. 324, No. 1, 01.12.2008, p. 99-107.

Research output: Contribution to journalArticle

El-Jouni, Wassim ; Haun, Shirley ; Machaca, Khaled. / Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation. In: Developmental Biology. 2008 ; Vol. 324, No. 1. pp. 99-107.
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