Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei

Bianca Kessler, Darawan Rinchai, Chidchamai Kewcharoenwong, Arnone Nithichanon, Rachael Biggart, Catherine M. Hawrylowicz, Gregory J. Bancroft, Ganjana Lertmemongkolchai

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Abstract

Melioidosis, caused by Burkholderia pseudomallei, is endemic in northeastern Thailand and Northern Australia. Severe septicemic melioidosis is associated with high levels of pro-inflammatory cytokines and is correlated with poor clinical outcomes. IL-10 is an immunoregulatory cytokine, which in other infections can control the expression of pro-inflammatory cytokines, but its role in melioidosis has not been addressed. Here, whole blood of healthy seropositive individuals (n = 75), living in N. E. Thailand was co-cultured with B. pseudomallei and production of IL-10 and IFN- 3 detected and the cellular sources identified. CD3 CD14 + monocytes were the main source of IL-10. Neutralization of IL-10 increased IFN- 3, IL-6 and TNF-α production and improved bacteria killing. IFN- 3 production and microbicidal activity were impaired in individuals with diabetes mellitus (DM). In contrast, IL-10 production was unimpaired in individuals with DM, resulting in an IL-10 dominant cytokine balance. Neutralization of IL-10 restored the IFN- 3 response of individuals with DM to similar levels observed in healthy individuals and improved killing of B. pseudomallei in vitro. These results demonstrate that monocyte derived IL-10 acts to inhibit potentially protective cell mediated immune responses against B. pseudomallei, but may also moderate the pathological effects of excessive cytokine production during sepsis.

Original languageEnglish
Article number42791
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 20 Feb 2017
Externally publishedYes

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Kessler, B., Rinchai, D., Kewcharoenwong, C., Nithichanon, A., Biggart, R., Hawrylowicz, C. M., Bancroft, G. J., & Lertmemongkolchai, G. (2017). Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Scientific Reports, 7, [42791]. https://doi.org/10.1038/srep42791