Interferon gamma and interferon gamma inducible protein-10 in detecting tuberculosis infection

Basirudeen S. Kabeer, Paulkumaran Paramasivam, Alamelu Raja

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: This study aimed to compare the levels of TB-antigen specific Interferon gamma (IFN-γ) and IFN-γ inducible protein (IP)-10 in culture of whole blood samples from healthy controls (HC) and healthy household contacts (HHC). Methodology: A total of 386 study subjects, which included 186 HC and 200 HHC, were recruited. QuantiFERON-TB Gold in-tube (QFT-IT) assay was employed to measure IFN-γ levels. IP-10 levels were measured in the supernatants collected from QFT-IT tubes. Tuberculin skin test was also performed. Results: The levels of TB antigen specific IFN-γ and IP-10 were significantly higher in HHC compared to HC. There was no significant difference observed between positivity of QFT-IT and IP-10 in HC and HHC. The positivity of TST was significantly lower in subjects <17 year, when compared to IP-10 (p< 0.005). The reduced cut-off point 0.22. IU/ml significantly increased the positivity of QFT-IT among children with high risk for latent TB infection (LTBI). Conclusions: Measurement of TB antigen specific IFN-γ and IP-10 can be potential markers for the detection of LTBI.

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalJournal of Infection
Volume64
Issue number6
DOIs
Publication statusPublished - Jun 2012
Externally publishedYes

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Keywords

  • Diagnosis
  • Interferon gamma inducible protein-10
  • QuantiFERON-TB Gold
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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