Abstract
Objective: This study aimed to compare the levels of TB-antigen specific Interferon gamma (IFN-γ) and IFN-γ inducible protein (IP)-10 in culture of whole blood samples from healthy controls (HC) and healthy household contacts (HHC). Methodology: A total of 386 study subjects, which included 186 HC and 200 HHC, were recruited. QuantiFERON-TB Gold in-tube (QFT-IT) assay was employed to measure IFN-γ levels. IP-10 levels were measured in the supernatants collected from QFT-IT tubes. Tuberculin skin test was also performed. Results: The levels of TB antigen specific IFN-γ and IP-10 were significantly higher in HHC compared to HC. There was no significant difference observed between positivity of QFT-IT and IP-10 in HC and HHC. The positivity of TST was significantly lower in subjects <17 year, when compared to IP-10 (p< 0.005). The reduced cut-off point 0.22. IU/ml significantly increased the positivity of QFT-IT among children with high risk for latent TB infection (LTBI). Conclusions: Measurement of TB antigen specific IFN-γ and IP-10 can be potential markers for the detection of LTBI.
| Original language | English |
|---|---|
| Pages (from-to) | 573-579 |
| Number of pages | 7 |
| Journal | Journal of Infection |
| Volume | 64 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2012 |
| Externally published | Yes |
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Keywords
- Diagnosis
- Interferon gamma inducible protein-10
- QuantiFERON-TB Gold
- Tuberculosis
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
Interferon gamma and interferon gamma inducible protein-10 in detecting tuberculosis infection. / Kabeer, Basirudeen S.; Paramasivam, Paulkumaran; Raja, Alamelu.
In: Journal of Infection, Vol. 64, No. 6, 06.2012, p. 573-579.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon gamma and interferon gamma inducible protein-10 in detecting tuberculosis infection
AU - Kabeer, Basirudeen S.
AU - Paramasivam, Paulkumaran
AU - Raja, Alamelu
PY - 2012/6
Y1 - 2012/6
N2 - Objective: This study aimed to compare the levels of TB-antigen specific Interferon gamma (IFN-γ) and IFN-γ inducible protein (IP)-10 in culture of whole blood samples from healthy controls (HC) and healthy household contacts (HHC). Methodology: A total of 386 study subjects, which included 186 HC and 200 HHC, were recruited. QuantiFERON-TB Gold in-tube (QFT-IT) assay was employed to measure IFN-γ levels. IP-10 levels were measured in the supernatants collected from QFT-IT tubes. Tuberculin skin test was also performed. Results: The levels of TB antigen specific IFN-γ and IP-10 were significantly higher in HHC compared to HC. There was no significant difference observed between positivity of QFT-IT and IP-10 in HC and HHC. The positivity of TST was significantly lower in subjects <17 year, when compared to IP-10 (p< 0.005). The reduced cut-off point 0.22. IU/ml significantly increased the positivity of QFT-IT among children with high risk for latent TB infection (LTBI). Conclusions: Measurement of TB antigen specific IFN-γ and IP-10 can be potential markers for the detection of LTBI.
AB - Objective: This study aimed to compare the levels of TB-antigen specific Interferon gamma (IFN-γ) and IFN-γ inducible protein (IP)-10 in culture of whole blood samples from healthy controls (HC) and healthy household contacts (HHC). Methodology: A total of 386 study subjects, which included 186 HC and 200 HHC, were recruited. QuantiFERON-TB Gold in-tube (QFT-IT) assay was employed to measure IFN-γ levels. IP-10 levels were measured in the supernatants collected from QFT-IT tubes. Tuberculin skin test was also performed. Results: The levels of TB antigen specific IFN-γ and IP-10 were significantly higher in HHC compared to HC. There was no significant difference observed between positivity of QFT-IT and IP-10 in HC and HHC. The positivity of TST was significantly lower in subjects <17 year, when compared to IP-10 (p< 0.005). The reduced cut-off point 0.22. IU/ml significantly increased the positivity of QFT-IT among children with high risk for latent TB infection (LTBI). Conclusions: Measurement of TB antigen specific IFN-γ and IP-10 can be potential markers for the detection of LTBI.
KW - Diagnosis
KW - Interferon gamma inducible protein-10
KW - QuantiFERON-TB Gold
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84860641969&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860641969&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2012.02.013
DO - 10.1016/j.jinf.2012.02.013
M3 - Article
VL - 64
SP - 573
EP - 579
JO - Journal of Infection
JF - Journal of Infection
SN - 0163-4453
IS - 6
ER -