Interferon-γ reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells

I. Caroline Le Poole, Adam I. Riker, M. Eugenia Quevedo, Lawrence S. Stennett, Ena Wang, Francesco M. Marincola, W. Martin Kast, June K. Robinson, Brian J. Nickoloff

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In malignant melanoma, tumor-infiltrating lymphocytes are frequently reactive with melanosomal antigens. Achieving complete remissions by peptide therapy is frequently hampered by metastases evading immune recognition. The tumor microenvironment seems to favor reduced expression of target antigens by melanoma cells. Among candidate factors, interferon-γ (IFN-γ) (102 to 103 U/ml) suppressed expression of antigens MART-1, TRP-1, and gp100 by M14 melanoma cells as shown by immunohistology and fluorescence-activated cell sorting analysis, reducing MART-1 expression by >65%. Northern blot analysis revealed that reduced expression was regulated at the transcriptional level, demonstrating a 79% reduction in MART-1 transcript abundance after 32 hours of IFN-γ treatment. To evaluate consequences of IFN-γ exposure for immune recognition, MART-1-responsive T cells were reacted with pretreated HLA-matched melanoma cells. Cytotoxicity was reduced up to 78% by IFN-γ pretreatment, and was restored by addition of MART-1 peptide AAGIGILTV for 2 hours. Examination of melanoma lesions by quantitative reverse transcriptase-polymerase chain reaction revealed up to 188-fold more abundant IFN-γ transcripts when compared to control skin. Laser capture microdissection and immunohistology localized most IFN-γ-producing T cells to the tumor stroma. Reduced MART-1 expression was frequently observed in adjacent tumor cells. Consequently, IFN-γ may enhance inflammatory responses yet hamper effective recognition of melanoma cells.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalAmerican Journal of Pathology
Volume160
Issue number2
Publication statusPublished - 2002
Externally publishedYes

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Interferons
Melanoma
T-Lymphocytes
Antigens
MART-1 Antigen
Laser Capture Microdissection
Melanoma-Specific Antigens
Tumor-Infiltrating Lymphocytes
Peptides
Tumor Microenvironment
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Interferon-gamma
Neoplasms
Flow Cytometry
Neoplasm Metastasis
Skin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Le Poole, I. C., Riker, A. I., Quevedo, M. E., Stennett, L. S., Wang, E., Marincola, F. M., ... Nickoloff, B. J. (2002). Interferon-γ reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells. American Journal of Pathology, 160(2), 521-528.

Interferon-γ reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells. / Le Poole, I. Caroline; Riker, Adam I.; Quevedo, M. Eugenia; Stennett, Lawrence S.; Wang, Ena; Marincola, Francesco M.; Kast, W. Martin; Robinson, June K.; Nickoloff, Brian J.

In: American Journal of Pathology, Vol. 160, No. 2, 2002, p. 521-528.

Research output: Contribution to journalArticle

Le Poole, IC, Riker, AI, Quevedo, ME, Stennett, LS, Wang, E, Marincola, FM, Kast, WM, Robinson, JK & Nickoloff, BJ 2002, 'Interferon-γ reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells', American Journal of Pathology, vol. 160, no. 2, pp. 521-528.
Le Poole, I. Caroline ; Riker, Adam I. ; Quevedo, M. Eugenia ; Stennett, Lawrence S. ; Wang, Ena ; Marincola, Francesco M. ; Kast, W. Martin ; Robinson, June K. ; Nickoloff, Brian J. / Interferon-γ reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells. In: American Journal of Pathology. 2002 ; Vol. 160, No. 2. pp. 521-528.
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AU - Marincola, Francesco M.

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