Interferon-α/β-mediated innate immune mechanisms in dermatomyositis

Steven A. Greenberg, Jack L. Pinkus, Geraldine S. Pinkus, Travis Burleson, Despina Sanoudou, Rabi Tawil, Richard J. Barohn, David S. Saperstein, Hannah R. Briemberg, Maria Ericsson, Peter Park, Anthony A. Amato

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    Abstract

    Dermatomyositis has been modeled as an autoimmune disease largely mediated by the adaptive immune system, including a local Immorally mediated response with B and T helper cell muscle infiltration, antibody and complement-mediated injury of capillaries, and perifascicular atrophy of muscle fibers caused by ischemia. To further understand the pathophysiology of dermatomyositis, we used microarrays, computational methods, immunohistochemistry and electron microscopy to study muscle specimens from 67 patients, 54 with inflammatory myopathies, 14 with dermatomyositis. In dermatomyositis, genes induced by interferon-α/ β were highly overexpressed, and immunohistochemistry for the interferon-α/β inducible protein MxA showed dense staining of perifascicular, and, sometimes all myofibers in 8/14 patients and on capillaries in 13/14 patients. Of 36 patients with other inflammatory myopathies, 1 patient had faint MxA staining of myofibers and 3 of capillaries. Plasmacytoid dendritic cells, potent CD4+ cellular sources of interferon-α, are present in substantial numbers in dermatomyositis and may account for most of the cells previously identified as T helper cells. In addition to an adaptive immune response, an innate immune response characterized by plasmacytoid dendritic cell infiltration and interferon-α/β inducible gene and protein expression may be an important part of the pathogenesis of dermatomyositis, as it appears to be in systemic lupus erythematosus.

    Original languageEnglish
    Pages (from-to)664-678
    Number of pages15
    JournalAnnals of Neurology
    Volume57
    Issue number5
    DOIs
    Publication statusPublished - 1 May 2005

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    ASJC Scopus subject areas

    • Neurology
    • Clinical Neurology

    Cite this

    Greenberg, S. A., Pinkus, J. L., Pinkus, G. S., Burleson, T., Sanoudou, D., Tawil, R., Barohn, R. J., Saperstein, D. S., Briemberg, H. R., Ericsson, M., Park, P., & Amato, A. A. (2005). Interferon-α/β-mediated innate immune mechanisms in dermatomyositis. Annals of Neurology, 57(5), 664-678. https://doi.org/10.1002/ana.20464